Thermo Scientific™ Phusion™ Plus DNA Polymerase

Description

• Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing
• It is a Pyrococcus -like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance
• Features include: >100X higher sequence accuracy than Taq polymerase Universal annealing temperature of 60°C in PCR Hot-start mechanism reduces non-specific amplification and primer degradation Automation enabled due to 24-hour stability of assembled reactions High inhibitor tolerance for work with DNA of suboptimal purity The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C
• This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs
• Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol
• Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature
• Enzyme activity is fully restored after the initial denaturation step
• Applications High-fidelity PCR Cloning & mutagenesis Template generation for sequencing GC-rich PCR Long-range PCR (up to 20 kb) High-throughput PCR Notes To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.