12-574-018
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™TaqDNA Polymerase
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-574-018-Each-of-1 | In Stock | ₹ 27,679.00 |
12-574-018 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,679.00
GST (18%): ₹ 4,982.22
Total Price: ₹ 32,661.22
Content And Storage
• SuperScript III RT/PlatinumTaqMix, 50 μL• 2X Reaction Mix, 1 mL• Magnesium Sulfate, 500 μL (5 mM)Store at –10 to –30°C.
Format
One-Step RT-PCR System
PCR Method
1-step RT-PCR
Reaction Speed
30 min.
Optimal Reaction Temperature
50°C
Ribonuclease H Activity
Reduced
Final Product Type
PCR Amplified cDNA
No. of Reactions
25 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Detection Method
Gel Electrophoresis
GC-Rich PCR Performance
High
Polymerase
Platinum Taq DNA Polymerase
Technique
1-Step RT-PCR
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
Hot Start
Built-In Hot Start
Reaction Format
Premixed Components
Size (Final Product)
Up to 4.5 kb
Related Products
Description
- The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR
- Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA
- The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb
- The amount of starting material can range from 0.01 pg to 1 μg of total RNA
- Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase • Sensitive detection down to 0.01 pg total RNA • Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability • SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers • Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility SuperScript III Reverse Transcriptase SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
- The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
- Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
- Platinum Taq DNA Polymerase Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures
- Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield
- Note: This kit has been optimized for end-point RT-PCR
- For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System
- Order Info Shipping Condition: Dry ice
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Content And Storage: • SuperScript III RT/PlatinumTaqMix, 50 μL• 2X Reaction Mix, 1 mL• Magnesium Sulfate, 500 μL (5 mM)Store at –10 to –30°C.
Format: One-Step RT-PCR System
PCR Method: 1-step RT-PCR
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Final Product Type: PCR Amplified cDNA
No. of Reactions: 25 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: Gel Electrophoresis
GC-Rich PCR Performance: High
Polymerase: Platinum Taq DNA Polymerase
Technique: 1-Step RT-PCR
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Reaction Format: Premixed Components
Size (Final Product): Up to 4.5 kb
Content And Storage:
• SuperScript III RT/PlatinumTaqMix, 50 μL• 2X Reaction Mix, 1 mL• Magnesium Sulfate, 500 μL (5 mM)Store at –10 to –30°C.
Format:
One-Step RT-PCR System
PCR Method:
1-step RT-PCR
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Final Product Type:
PCR Amplified cDNA
No. of Reactions:
25 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
Gel Electrophoresis
GC-Rich PCR Performance:
High
Polymerase:
Platinum Taq DNA Polymerase
Technique:
1-Step RT-PCR
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Reaction Format:
Premixed Components
Size (Final Product):
Up to 4.5 kb
Content And Storage: • SuperScript III RT/PlatinumTaqMix (200 μL)• 2X Reaction Mix (3 x 1 mL)• 5 mM Magnesium Sulfate (500 μL)Store at –10 to –30°C.
Format: One-Step RT-PCR System
PCR Method: 1-step RT-PCR
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Final Product Type: PCR Amplified cDNA
No. of Reactions: 100 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: Gel Electrophoresis
GC-Rich PCR Performance: High
Polymerase: Platinum Taq DNA Polymerase
Technique: 1-Step RT-PCR
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Reaction Format: Premixed Components
Size (Final Product): Up to 4.5 kb
Content And Storage:
• SuperScript III RT/PlatinumTaqMix (200 μL)• 2X Reaction Mix (3 x 1 mL)• 5 mM Magnesium Sulfate (500 μL)Store at –10 to –30°C.
Format:
One-Step RT-PCR System
PCR Method:
1-step RT-PCR
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Final Product Type:
PCR Amplified cDNA
No. of Reactions:
100 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
Gel Electrophoresis
GC-Rich PCR Performance:
High
Polymerase:
Platinum Taq DNA Polymerase
Technique:
1-Step RT-PCR
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Reaction Format:
Premixed Components
Size (Final Product):
Up to 4.5 kb
Content And Storage: • SuperScript III RT/PlatinumTaqHigh Fidelity Enzyme Mix (25 μL)• 2X Reaction Mix (1 mL)• 5 mM Magnesium Sulfate (500 μL)Store at –10 to –30°C.
Format: One-Step RT-PCR System
PCR Method: 1-step RT-PCR
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Final Product Type: PCR Amplified cDNA
No. of Reactions: 25 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: Gel Electrophoresis
GC-Rich PCR Performance: High
Polymerase: Platinum Taq High Fidelity
Technique: 1-Step RT-PCR
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Reaction Format: Premixed Components
Size (Final Product): Up to 10 kb
Content And Storage:
• SuperScript III RT/PlatinumTaqHigh Fidelity Enzyme Mix (25 μL)• 2X Reaction Mix (1 mL)• 5 mM Magnesium Sulfate (500 μL)Store at –10 to –30°C.
Format:
One-Step RT-PCR System
PCR Method:
1-step RT-PCR
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Final Product Type:
PCR Amplified cDNA
No. of Reactions:
25 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
Gel Electrophoresis
GC-Rich PCR Performance:
High
Polymerase:
Platinum Taq High Fidelity
Technique:
1-Step RT-PCR
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Reaction Format:
Premixed Components
Size (Final Product):
Up to 10 kb
Content And Storage: • SuperScript III RT/PlatinumTaqHigh Fidelity Enzyme Mix (100 μL)• 2X Reaction Mix (3 x 1 mL)• 5 mM Magnesium Sulfate (500 μL)Store at –10 to –30°C.
Format: One-Step RT-PCR System
PCR Method: 1-step RT-PCR
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Final Product Type: PCR Amplified cDNA
No. of Reactions: 100 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: Gel Electrophoresis
GC-Rich PCR Performance: High
Polymerase: Platinum Taq High Fidelity
Technique: 1-Step RT-PCR
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Reaction Format: Premixed Components
Size (Final Product): Up to 10 kb
Content And Storage:
• SuperScript III RT/PlatinumTaqHigh Fidelity Enzyme Mix (100 μL)• 2X Reaction Mix (3 x 1 mL)• 5 mM Magnesium Sulfate (500 μL)Store at –10 to –30°C.
Format:
One-Step RT-PCR System
PCR Method:
1-step RT-PCR
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Final Product Type:
PCR Amplified cDNA
No. of Reactions:
100 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
Gel Electrophoresis
GC-Rich PCR Performance:
High
Polymerase:
Platinum Taq High Fidelity
Technique:
1-Step RT-PCR
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Reaction Format:
Premixed Components
Size (Final Product):
Up to 10 kb