Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™TaqDNA Polymerase

Description

• The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR
• Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA
• The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb
• The amount of starting material can range from 0.01 pg to 1 μg of total RNA
• Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase • Sensitive detection down to 0.01 pg total RNA • Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability • SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers • Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility SuperScript III Reverse Transcriptase SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
• The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
• Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
• Platinum Taq DNA Polymerase Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures
• Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield
• Note: This kit has been optimized for end-point RT-PCR
• For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System
• Order Info Shipping Condition: Dry ice