18-010-025

Invitrogen™ DNA Polymerase I

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 18-010-025-Each-of-1 In Stock ₹ 47,058.00

18-010-025 - Each of 1

₹ 47,058.00

In Stock

Quantity

1

Base Price: ₹ 47,058.00

GST (18%): ₹ 8,470.44

Total Price: ₹ 55,528.44

Content And Storage

1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μl.Store at -5°C to -30°C.

Polymerase

DNA Polymerase I

Quantity

1000 U

Hot Start

No

Description

DNA Polymerase

Exonuclease Activity

3' - 5', 5' - 3'

Shipping Condition

Wet Ice

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Description

  • Performance and quality testing Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined
  • Unit definition One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer
  • Unit reaction conditions 50mM potassium phosphate (pH 7.0), 6.7mM MgCl 2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [ 3H ]dTTP, and enzyme in 100μL for 30 min at 37°C
  • Also incorporates biotinylated nucleotides
  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs Source: purified from E
  • coli expressing the DNA Polymerase I gene on a plasmid Cloning, Restriction Enzyme Cloning Order Info Shipping Condition: Wet Ice

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Fischer Scientific

18-010-025

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Content And Storage:
1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μl.Store at -5°C to -30°C.

Polymerase:
DNA Polymerase I

Quantity:
1000 U

Hot Start:
No

Description:
DNA Polymerase

Exonuclease Activity:
3' - 5', 5' - 3'

Shipping Condition:
Wet Ice

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Content And Storage:
Bacterial Alkaline Phosphatase is supplied with a vial of 10X dephosphorylation buffer [100 mM Tris-HCl (pH 8.0)]. Store at -20°C.

Polymerase:
__

Quantity:
2500 U

Hot Start:
__

Description:
__

Exonuclease Activity:
__

Shipping Condition:
Wet Ice

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Content And Storage:
DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mMMgCl2, 500 mM NaCl], vial of dilution buffer. Store at -20°C.

Polymerase:
DNA Polymerase I

Quantity:
100 Units

Hot Start:
No

Description:
DNA Polymerase

Exonuclease Activity:
3' to 5'

Shipping Condition:
Approved for shipment on Wet or Dry Ice

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Content And Storage:
DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mMMgCl2, 500 mM NaCl], vial of dilution buffer. Store at -20°C.

Polymerase:
DNA Polymerase I

Quantity:
500 Units

Hot Start:
No

Description:
DNA Polymerase

Exonuclease Activity:
3' to 5'

Shipping Condition:
Approved for shipment on Wet or Dry Ice