43-041-34
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-041-34-Each-of-1 | In Stock | ₹ 6,73,357.20 |
43-041-34 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 6,73,357.20
GST (18%): ₹ 1,21,204.296
Total Price: ₹ 7,94,561.496
Content And Storage
Includes 10 units of Cat. No. N8080234. Each unit contains:• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)• RNase Inhibitor, 100 μL (20 U/μL)• dNTP Mixture, 1 mL (2.5 mM each dNTP)• Oligo (dT)16, 100 μL (50 μM)• Random Hexamers, 100 μL (50 μM)• 10X RT Buffer, 1.5 mL• MgCl2Solution, 1.5 mLStore at –15 to –25°C.
Technique
Reverse Transcription
Reverse Transcriptase
MultiScribe
Shipping Condition
Dry Ice
Concentration
50 U/μL
No. of Reactions
2,000 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Kit
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Yes
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 5 kb
Related Products
Description
- TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR)
- Random hexamers, oligo (dT) 16 , and reverse primers are included for cDNA synthesis flexibility
- These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA
- The reagents in this kit facilitate two-step RT-PCR
- Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA
- Advantages of TaqMan Reverse Transcription Reagents • Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT) • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay setup • Compatible with two-step RT-PCR reactions Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA
- For two-step RT-PCR, random hexamers, oligo (dT) 16 , and sequence-specific reverse primers can all be used
- The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
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Content And Storage: Includes 10 units of Cat. No. N8080234. Each unit contains:• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)• RNase Inhibitor, 100 μL (20 U/μL)• dNTP Mixture, 1 mL (2.5 mM each dNTP)• Oligo (dT)16, 100 μL (50 μM)• Random Hexamers, 100 μL (50 μM)• 10X RT Buffer, 1.5 mL• MgCl2Solution, 1.5 mLStore at –15 to –25°C.
Technique: Reverse Transcription
Reverse Transcriptase: MultiScribe
Shipping Condition: Dry Ice
Concentration: 50 U/μL
No. of Reactions: 2,000 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Yes
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 5 kb
Content And Storage:
Includes 10 units of Cat. No. N8080234. Each unit contains:• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)• RNase Inhibitor, 100 μL (20 U/μL)• dNTP Mixture, 1 mL (2.5 mM each dNTP)• Oligo (dT)16, 100 μL (50 μM)• Random Hexamers, 100 μL (50 μM)• 10X RT Buffer, 1.5 mL• MgCl2Solution, 1.5 mLStore at –15 to –25°C.
Technique:
Reverse Transcription
Reverse Transcriptase:
MultiScribe
Shipping Condition:
Dry Ice
Concentration:
50 U/μL
No. of Reactions:
2,000 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Yes
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 5 kb
Content And Storage: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
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Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Content And Storage:
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Technique:
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Reverse Transcriptase:
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Shipping Condition:
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Concentration:
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No. of Reactions:
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Reagent Type:
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Starting Material:
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Format:
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Optimal Reaction Temperature:
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Ribonuclease H Activity:
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For Use With (Application):
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Final Product Type:
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Reaction Format:
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Size (Final Product):
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Content And Storage: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Content And Storage:
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Technique:
__
Reverse Transcriptase:
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Shipping Condition:
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Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Content And Storage: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Content And Storage:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__