N8080234
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | N8080234-Each-of-1 | In Stock | ₹ 72,624.00 |
N8080234 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 72,624.00
GST (18%): ₹ 13,072.32
Total Price: ₹ 85,696.32
Content And Storage
• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)• RNase Inhibitor, 100 μL (20 U/μL)• dNTP Mixture, 1 mL (2.5 mM each dNTP)• Oligo (dT)16, 100 μL (50 μM)• Random Hexamers, 100 μL (50 μM)• 10X RT Buffer, 1.5 mL• MgCl2Solution, 1.5 mLStore at –15 to –25°C.
Technique
Reverse Transcription
Reverse Transcriptase
MultiScribe
Shipping Condition
Dry Ice
Concentration
50 U/μL
No. of Reactions
200 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Kit
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Yes
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 5 kb
Related Products
Description
- TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR)
- Random hexamers, oligo (dT) 16 , and reverse primers are included for cDNA synthesis flexibility
- These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA
- The reagents in this kit facilitate two-step RT-PCR
- Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA
- Advantages of TaqMan Reverse Transcription Reagents • Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT) • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay setup • Compatible with two-step RT-PCR reactions Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA
- For two-step RT-PCR, random hexamers, oligo (dT) 16 , and sequence-specific reverse primers can all be used
- The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
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Content And Storage: • MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)• RNase Inhibitor, 100 μL (20 U/μL)• dNTP Mixture, 1 mL (2.5 mM each dNTP)• Oligo (dT)16, 100 μL (50 μM)• Random Hexamers, 100 μL (50 μM)• 10X RT Buffer, 1.5 mL• MgCl2Solution, 1.5 mLStore at –15 to –25°C.
Technique: Reverse Transcription
Reverse Transcriptase: MultiScribe
Shipping Condition: Dry Ice
Concentration: 50 U/μL
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Yes
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 5 kb
Content And Storage:
• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)• RNase Inhibitor, 100 μL (20 U/μL)• dNTP Mixture, 1 mL (2.5 mM each dNTP)• Oligo (dT)16, 100 μL (50 μM)• Random Hexamers, 100 μL (50 μM)• 10X RT Buffer, 1.5 mL• MgCl2Solution, 1.5 mLStore at –15 to –25°C.
Technique:
Reverse Transcription
Reverse Transcriptase:
MultiScribe
Shipping Condition:
Dry Ice
Concentration:
50 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Yes
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 5 kb
Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL• 10X PCR Buffer I (with MgCl2), 1 x 1.5 mLStore at -15°C to -30°C.
Technique: __
Reverse Transcriptase: __
Shipping Condition: Dry Ice
Concentration: 5 U/μL
No. of Reactions: 200 Reactions
Reagent Type: __
Starting Material: DNA
Format: Tube
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): Hot-start PCR
Final Product Type: __
Reaction Format: Separate Components
Size (Final Product): 5 kb or less
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL• 10X PCR Buffer I (with MgCl2), 1 x 1.5 mLStore at -15°C to -30°C.
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
__
Starting Material:
DNA
Format:
Tube
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
Hot-start PCR
Final Product Type:
__
Reaction Format:
Separate Components
Size (Final Product):
5 kb or less
Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL• 10X PCR Buffer II, 1 x 1.5 mL• 25 mM MgCl2, 1 x 1.5 mLStore at -15°C to -30°C.
Technique: __
Reverse Transcriptase: __
Shipping Condition: Dry Ice
Concentration: 5 U/μL
No. of Reactions: 200 Reactions
Reagent Type: __
Starting Material: DNA
Format: Tube
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): Hot-start PCR
Final Product Type: __
Reaction Format: Separate Components
Size (Final Product): 5 kb or less
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL• 10X PCR Buffer II, 1 x 1.5 mL• 25 mM MgCl2, 1 x 1.5 mLStore at -15°C to -30°C.
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
__
Starting Material:
DNA
Format:
Tube
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
Hot-start PCR
Final Product Type:
__
Reaction Format:
Separate Components
Size (Final Product):
5 kb or less
Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 12 x 50 μL• 10X PCR Buffer I (with MgCl2), 12 x 1.5 mLStore at -15°C to -30°C.
Technique: __
Reverse Transcriptase: __
Shipping Condition: Dry Ice
Concentration: 5 U/μL
No. of Reactions: 2400 Reactions
Reagent Type: __
Starting Material: DNA
Format: Tube
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): Hot-start PCR
Final Product Type: __
Reaction Format: Separate Components
Size (Final Product): 5 kb or less
Content And Storage:
• AmpliTaq Gold DNA Polymerase (5 U/μL), 12 x 50 μL• 10X PCR Buffer I (with MgCl2), 12 x 1.5 mLStore at -15°C to -30°C.
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
Dry Ice
Concentration:
5 U/μL
No. of Reactions:
2400 Reactions
Reagent Type:
__
Starting Material:
DNA
Format:
Tube
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
Hot-start PCR
Final Product Type:
__
Reaction Format:
Separate Components
Size (Final Product):
5 kb or less