Thermo Scientific™ Verso cDNA Synthesis Kit

Description

• The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool
• The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system
• The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure
• The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool
• Features of the Verso cDNA Synthesis Kit include: • Highly sensitive with a broad dynamic range—can reverse transcribe RNA from an input range of 1pg to 1μg • Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes) • Unique priming strategy for increased efficiency and broad template flexibility • High affinity RT enzyme effectively transcribes through difficult RNA sequences • Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates • Included RT enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment • RNase inhibitor included • Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR Applications • 2-step RT-(q)PCR • Single stranded cDNA synthesis