Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Description

• Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates
• The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity
• Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA
• Features of the RevertAid H Minus First Strand cDNA Synthesis Kit include: • High yields of full-length first-strand cDNA up to 13 kb • Increased reaction temperatures of 42–55°C • Supplied with the recombinant RiboLock RNase Inhibitor • Complete kit includes oligo(dT) 18 and random hexamer primers Applications • First strand cDNA synthesis for RT-PCR and RT-qPCR • Construction of full length cDNA libraries • Antisense RNA synthesis Additional Features The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation
• It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C
• The kit is supplied with both oligo(dT) 18 and random hexamer primers
• The oligo(dT) 18 primer anneals selectively to the poly(A) tail of mRNA
• Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs
• Gene-specific primers may also be used with the kit
• The first strand cDNA can be directly used as a template in PCR, real-time PCR, or in second strand cDNA synthesis.