FERK1622
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK1622-Each-of-1 | In Stock | ₹ 55,981.00 |
FERK1622 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,981.00
GST (18%): ₹ 10,076.58
Total Price: ₹ 66,057.58
Content And Storage
• RevertAid Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control GAPDH RNA• Forward GAPDH Primer (10 μM)• Reverse GAPDH Primer (10 μM)• Nuclease-free waterStore at –20°C.
Reaction Speed
60 min.
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Yes
For Use With (Application)
RT-PCR
No. of Reactions
100 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Kit
Technique
Reverse Transcription
Reverse Transcriptase
RevertAid
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 13 kb
Related Products
Description
- Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates
- The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb
- RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation at temperatures up to 55°C
- The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT) 18 and random hexamer primers
- The oligo(dT) 18 primer anneals selectively to the poly(A) tail of mRNA
- Random hexamer primers do not require the presence of the poly(A) tail; they can therefore be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs)
- Gene-specific primers may also be used with the kit
- Radioactively- and non-radioactively-labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays
- Features of the RevertAid First Strand cDNA Synthesis Kit include: • Full-length first strand cDNA up to 13 kb • Optimum reaction temperature 42°C • Complete kit with all components necessary for the RT reaction already included Applications • First strand cDNA synthesis for RT-PCR and RT-qPCR • Construction of full length cDNA libraries • Antisense RNA synthesis
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Content And Storage: • RevertAid Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control GAPDH RNA• Forward GAPDH Primer (10 μM)• Reverse GAPDH Primer (10 μM)• Nuclease-free waterStore at –20°C.
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Yes
For Use With (Application): RT-PCR
No. of Reactions: 100 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Technique: Reverse Transcription
Reverse Transcriptase: RevertAid
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 13 kb
Content And Storage:
• RevertAid Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control GAPDH RNA• Forward GAPDH Primer (10 μM)• Reverse GAPDH Primer (10 μM)• Nuclease-free waterStore at –20°C.
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Yes
For Use With (Application):
RT-PCR
No. of Reactions:
100 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Technique:
Reverse Transcription
Reverse Transcriptase:
RevertAid
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 13 kb
Content And Storage: • RevertAid H Minus Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control RNA• Control Primers• Nuclease-free waterStore at –20°C.
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C to 45°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR), RT-PCR
No. of Reactions: 20 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Technique: Reverse Transcription
Reverse Transcriptase: RevertAid H Minus
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 13 kb
Content And Storage:
• RevertAid H Minus Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control RNA• Control Primers• Nuclease-free waterStore at –20°C.
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C to 45°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR), RT-PCR
No. of Reactions:
20 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Technique:
Reverse Transcription
Reverse Transcriptase:
RevertAid H Minus
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 13 kb
Content And Storage: • RevertAid H Minus Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control RNA• Control Primers• Nuclease-free waterStore at –20°C.
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C to 45°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR), RT-PCR
No. of Reactions: 100 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Technique: Reverse Transcription
Reverse Transcriptase: RevertAid H Minus
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 13 kb
Content And Storage:
• RevertAid H Minus Reverse Transcriptase• RiboLock RNase Inhibitor• 5X Reaction Buffer• dNTP Mix• Oligo(dT)18Primer• Random Hexamer Primer• Control RNA• Control Primers• Nuclease-free waterStore at –20°C.
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C to 45°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR), RT-PCR
No. of Reactions:
100 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Technique:
Reverse Transcription
Reverse Transcriptase:
RevertAid H Minus
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 13 kb
Content And Storage: • Maxima Enzyme Mix• 5X Reaction Mix• Nuclease-free waterStore at –20°C.
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C to 55°C
Ribonuclease H Activity: Yes
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Technique: Reverse Transcription
Reverse Transcriptase: Maxima
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 20 kb
Content And Storage:
• Maxima Enzyme Mix• 5X Reaction Mix• Nuclease-free waterStore at –20°C.
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C to 55°C
Ribonuclease H Activity:
Yes
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Technique:
Reverse Transcription
Reverse Transcriptase:
Maxima
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 20 kb