Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

Description

• The Applied Biosystems™ TaqMan™ RNase P Detection Reagents kit provides the components needed to detect and quantitate genomic copies of the human RNase P gene using the 5' nuclease assay
• Human RNase P gene is a single-copy gene encoding the RNA moiety for the RNase P enzyme
• This 5' nuclease assay employs TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR) using the provided human genomic DNA as template
• Pre-designed primers and TaqMan™ probe eliminate assay design Easy-to-use primer and TaqMan™ probe mix minimizes assay set-up time Rapid Assay Development Guidelines can minimize optimization time Comprehensive Guidelines Included Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation
• These guidelines are simple and easy to follow
• Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time
• Normalization Built-In All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations
• Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation
• The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube
• Note: See user's manual or package insert for limited label license, and trademark information
• For Research Use Only
• Not for use in diagnostics procedures.