Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P

Description

• Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction Used to detect and measure copy number variations (CNVs) and smaller regions in the human genome Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT Designed to unique genomic sequences in the reference genome assembly (build GRCh38) and are required for relative quantitation of copy number targets TaqMan™ Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome Please note that TaqMan™ Copy Number Reference Assays are species specific
• Assays should be stored at -15°C to -25°C in the dark Minimize freeze-thaw cycles TaqMan™ Copy Number Reference Assay RNase P Recommended as the standard reference assay for copy number analysis This assay detects the Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2 The assay location is chr.14:20343370 on build GRCh38 It has an 87 bp amplicon that maps within the single exon RPPH1 gene TaqMan™ Copy Number Reference Assay TERT An alternative reference assay recommended in the event that the RNase P assay functions poorly with a sample This could be due to chromosomal aberrations or other issues This assay targets the telomerase reverse transcriptase (TERT) gene located on chromosome 5, cytoband 5p15.33 The assay location is chr5:1253257 on build GRCh38 It has an 88 bp amplicon that maps within exon 16 of the TERT gene