43-444-63
Applied Biosystems™ SYBR™ Green Universal Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-444-63-Each-of-1 | In Stock | ₹ 13,518.48 |
43-444-63 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 13,518.48
GST (18%): ₹ 2,433.326
Total Price: ₹ 15,951.806
Content And Storage
SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Form
Concentrated
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold DNA Polymerase
Technique
Fluorescence Intensity
Label or Dye
SYBR Green
Product Line
SYBR™
Quantity
1 x 1 mL
Sufficient For
40 Reactions of 50 μL
For Use With (Application)
Gene Expression
Hot Start
Built-In Hot Start
Sample Type
DNA
Detection Method
SYBR
Format
Tube
PCR Method
qPCR
Reaction Speed
Standard
For Use With (Equipment)
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700
Passive Reference Dye
ROX (Pre-mixed)
Product Type
Real Time PCR SYBR Master Mix
Shipping Condition
Wet Ice
Target Specificity
Not Target-Specific
Concentration
2X
No. of Reactions
40 Reactions
Volume
1 x 1 mL
Related Products
Description
- Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase Proprietary buffer enhancements ensure performance and reliability Premixed components stored at 2° to 8°C significantly reduce assay setup time Maximum Flexibility and Convenience: Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed
- Store at -20°C Order Info Shipping Condition: Wet Ice
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Content And Storage: SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Form: Concentrated
GC-Rich PCR Performance: High
Polymerase: AmpliTaq Gold DNA Polymerase
Technique: Fluorescence Intensity
Label or Dye: SYBR Green
Product Line: SYBR™
Quantity: 1 x 1 mL
Sufficient For: 40 Reactions of 50 μL
For Use With (Application): Gene Expression
Hot Start: Built-In Hot Start
Sample Type: DNA
Detection Method: SYBR
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700
Passive Reference Dye: ROX (Pre-mixed)
Product Type: Real Time PCR SYBR Master Mix
Shipping Condition: Wet Ice
Target Specificity: Not Target-Specific
Concentration: 2X
No. of Reactions: 40 Reactions
Volume: 1 x 1 mL
Content And Storage:
SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Form:
Concentrated
GC-Rich PCR Performance:
High
Polymerase:
AmpliTaq Gold DNA Polymerase
Technique:
Fluorescence Intensity
Label or Dye:
SYBR Green
Product Line:
SYBR™
Quantity:
1 x 1 mL
Sufficient For:
40 Reactions of 50 μL
For Use With (Application):
Gene Expression
Hot Start:
Built-In Hot Start
Sample Type:
DNA
Detection Method:
SYBR
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
For Use With (Equipment):
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700
Passive Reference Dye:
ROX (Pre-mixed)
Product Type:
Real Time PCR SYBR Master Mix
Shipping Condition:
Wet Ice
Target Specificity:
Not Target-Specific
Concentration:
2X
No. of Reactions:
40 Reactions
Volume:
1 x 1 mL
Content And Storage: __
Form: __
GC-Rich PCR Performance: __
Polymerase: __
Technique: __
Label or Dye: __
Product Line: __
Quantity: __
Sufficient For: __
For Use With (Application): __
Hot Start: __
Sample Type: __
Detection Method: __
Format: __
PCR Method: __
Reaction Speed: __
For Use With (Equipment): __
Passive Reference Dye: __
Product Type: __
Shipping Condition: __
Target Specificity: __
Concentration: __
No. of Reactions: __
Volume: __
Content And Storage:
__
Form:
__
GC-Rich PCR Performance:
__
Polymerase:
__
Technique:
__
Label or Dye:
__
Product Line:
__
Quantity:
__
Sufficient For:
__
For Use With (Application):
__
Hot Start:
__
Sample Type:
__
Detection Method:
__
Format:
__
PCR Method:
__
Reaction Speed:
__
For Use With (Equipment):
__
Passive Reference Dye:
__
Product Type:
__
Shipping Condition:
__
Target Specificity:
__
Concentration:
__
No. of Reactions:
__
Volume:
__
Content And Storage: Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Form: Liquid
GC-Rich PCR Performance: __
Polymerase: __
Technique: __
Label or Dye: __
Product Line: __
Quantity: 100 μL
Sufficient For: __
For Use With (Application): __
Hot Start: __
Sample Type: __
Detection Method: __
Format: __
PCR Method: __
Reaction Speed: __
For Use With (Equipment): __
Passive Reference Dye: __
Product Type: Antibody
Shipping Condition: __
Target Specificity: __
Concentration: 1 mg/mL
No. of Reactions: __
Volume: __
Content And Storage:
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Form:
Liquid
GC-Rich PCR Performance:
__
Polymerase:
__
Technique:
__
Label or Dye:
__
Product Line:
__
Quantity:
100 μL
Sufficient For:
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For Use With (Application):
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Hot Start:
__
Sample Type:
__
Detection Method:
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Format:
__
PCR Method:
__
Reaction Speed:
__
For Use With (Equipment):
__
Passive Reference Dye:
__
Product Type:
Antibody
Shipping Condition:
__
Target Specificity:
__
Concentration:
1 mg/mL
No. of Reactions:
__
Volume:
__
Content And Storage: __
Form: __
GC-Rich PCR Performance: __
Polymerase: __
Technique: __
Label or Dye: __
Product Line: __
Quantity: 8 runs
Sufficient For: __
For Use With (Application): __
Hot Start: __
Sample Type: __
Detection Method: __
Format: __
PCR Method: __
Reaction Speed: __
For Use With (Equipment): SeqStudio 3500 and 3730xl
Passive Reference Dye: __
Product Type: __
Shipping Condition: Wet Ice
Target Specificity: __
Concentration: __
No. of Reactions: __
Volume: __
Content And Storage:
__
Form:
__
GC-Rich PCR Performance:
__
Polymerase:
__
Technique:
__
Label or Dye:
__
Product Line:
__
Quantity:
8 runs
Sufficient For:
__
For Use With (Application):
__
Hot Start:
__
Sample Type:
__
Detection Method:
__
Format:
__
PCR Method:
__
Reaction Speed:
__
For Use With (Equipment):
SeqStudio 3500 and 3730xl
Passive Reference Dye:
__
Product Type:
__
Shipping Condition:
Wet Ice
Target Specificity:
__
Concentration:
__
No. of Reactions:
__
Volume:
__