4391178

Applied Biosystems™PowerSYBR™ Green RNA-to-CT™1-StepKit

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 4391178-Each-of-1 In Stock ₹ 20,363.28

4391178 - Each of 1

₹ 20,363.28

In Stock

Quantity

1

Base Price: ₹ 20,363.28

GST (18%): ₹ 3,665.39

Total Price: ₹ 24,028.67

Content And Storage

Sufficient for 40 reactions at 50 μL reaction volume and includes:• 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.• 1 x 20 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Format

Tube

Polymerase

AmpliTaq Gold DNA Polymerase

For Use With (Equipment)

7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System

Label or Dye

SYBR Green

Product Line

RNA-to-CT™, SYBR™

Purity or Quality Grade

UP (Ultra Pure)

Reverse Transcriptase

ArrayScript™ UP

Shipping Condition

Wet Ice

For Use With (Application)

Gene Expression

No. of Reactions

40 Reactions

Volume

1 x 1 mL

Detection Method

SYBR

PCR Method

1-step RT-qPCR

Reaction Speed

Standard

Kit Type

Green RNA-to-CT 1-Step Kit

Passive Reference Dye

ROX (Pre-mixed)

Product Type

Real Time PCR SYBR Master Mix

Quantity

1 x 1 mL

Run Time

Standard

Sufficient For

40 Reactions at 50 μL

Concentration

2X (MasterMix), 125X (RT Enzyme Mix)

Sample Type

RNA

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Description

  • The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets
  • Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction
  • Formulated to ensure maximum sensitivity and reliability Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets Includes: AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals RNase inhibitor, to prevent degradation of RNA templates A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

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Fischer Scientific

4391178

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Content And Storage:
Sufficient for 40 reactions at 50 μL reaction volume and includes:• 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.• 1 x 20 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Format:
Tube

Polymerase:
AmpliTaq Gold DNA Polymerase

For Use With (Equipment):
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System

Label or Dye:
SYBR Green

Product Line:
RNA-to-CT™, SYBR™

Purity or Quality Grade:
UP (Ultra Pure)

Reverse Transcriptase:
ArrayScript™ UP

Shipping Condition:
Wet Ice

For Use With (Application):
Gene Expression

No. of Reactions:
40 Reactions

Volume:
1 x 1 mL

Detection Method:
SYBR

PCR Method:
1-step RT-qPCR

Reaction Speed:
Standard

Kit Type:
Green RNA-to-CT 1-Step Kit

Passive Reference Dye:
ROX (Pre-mixed)

Product Type:
Real Time PCR SYBR Master Mix

Quantity:
1 x 1 mL

Run Time:
Standard

Sufficient For:
40 Reactions at 50 μL

Concentration:
2X (MasterMix), 125X (RT Enzyme Mix)

Sample Type:
RNA

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