50-591-795

Applied Biosystems™PowerSYBR™ Green RNA-to-CT™1-StepKit

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 50-591-795-Each-of-1 In Stock ₹ 71,870.40

50-591-795 - Each of 1

₹ 71,870.40

In Stock

Quantity

1

Base Price: ₹ 71,870.40

GST (18%): ₹ 12,936.672

Total Price: ₹ 84,807.072

Content And Storage

Sufficient for 200 reactions at 50 μL reaction volume and includes:• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

PCR Method

1-step RT-qPCR

Reaction Speed

Standard

Label or Dye

SYBR Green

Product Line

RNA-to-CT™, SYBR™

Purity or Quality Grade

UP (Ultra Pure)

Run Time

Standard

Sufficient For

200 Reactions

No. of Reactions

200 Reactions

Detection Method

SYBR

Polymerase

AmpliTaq Gold DNA Polymerase

For Use With (Equipment)

7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System

Passive Reference Dye

ROX (Pre-mixed)

Product Type

One-Step qRT-PCR Kit

Reverse Transcriptase

ArrayScript™ UP

Shipping Condition

Wet Ice

For Use With (Application)

Gene Expression

Sample Type

RNA

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Description

  • The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets
  • Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction
  • Formulated to ensure maximum sensitivity and reliability Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets Includes: AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals RNase inhibitor, to prevent degradation of RNA templates A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

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Fischer Scientific

50-591-795

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Content And Storage:
Sufficient for 200 reactions at 50 μL reaction volume and includes:• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

PCR Method:
1-step RT-qPCR

Reaction Speed:
Standard

Label or Dye:
SYBR Green

Product Line:
RNA-to-CT™, SYBR™

Purity or Quality Grade:
UP (Ultra Pure)

Run Time:
Standard

Sufficient For:
200 Reactions

No. of Reactions:
200 Reactions

Detection Method:
SYBR

Polymerase:
AmpliTaq Gold DNA Polymerase

For Use With (Equipment):
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System

Passive Reference Dye:
ROX (Pre-mixed)

Product Type:
One-Step qRT-PCR Kit

Reverse Transcriptase:
ArrayScript™ UP

Shipping Condition:
Wet Ice

For Use With (Application):
Gene Expression

Sample Type:
RNA

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Content And Storage:
Contains 1 vial of Zenon APC mouse IgG1labeling reagent (125 μL), and 1 vial of Zenon blocking reagent (mouse IgG, 125 μL).Store in refrigerator (2–8°C) and protect from light.

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APC (Allophycocyanin)

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Zenon™

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Selleck Chemical LLC

50-592-0

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Selleck Chemical LLC

50-592-2

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