44-643-35
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
Manufacturer: Applied Biosystems™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 44-643-35-Each-of-1 | In Stock | ₹ 3,03,738.00 |
44-643-35 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,03,738.00
GST (18%): ₹ 54,672.84
Total Price: ₹ 3,58,410.84
Content And Storage
resDNASEQ MDCK DNA Control• MDCK DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ MDCK DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• MDCK DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format
Kit
Host Cell
MDCK
Product Line
resDNASEQ™
No. of Reactions
100 Reactions
Detection Method
Primer-probe
PCR Method
qPCR
Label or Dye
FAM
Shipping Condition
Dry Ice
Includes
Assay Kit only
Related Products
Description
- The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines
- Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA
- This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, if the sample contains high molecular weight or sheared DNA
- Features of the resDNASEQ Quantitative MDCK DNA Kit include: • Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology • Consistent performance across the expected range of DNA fragment sizes • Includes master mix, TaqMan™ primer/probe mix, and MDCK genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
- The resDNASEQ™ Quantitative MDCK DNA Kit is designed to provide highly sensitive detection of MDCK DNA, allowing you to use small sample volumes to generate accurate results
- The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of MDCK DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts
- Easy to use With the resDNASEQ™ Quantitative MDCK DNA Kit you perform qPCR analysis to compare the amount of MDCK DNA in your test samples to a standard curve generated with known amounts of purified MDCK genomic DNA standard
- The resDNASEQ™ kit includes almost everything you need to carry out these steps, from master mix to TaqMan™ primer/probe mix to MDCK genomic DNA standard, as well as step-by-step instructions
- Specific to MDCK DNA, no cross-reactivity with unrelated DNA The target of the assay is highly specific—it only detects a MDCK-specific region of a multicopy genetic element
- We selected this region using extensive bioinformatic analysis of multiple related and unrelated species
- Consistent performance even with fragmented DNA For accurate quantitation of residual MDCK DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample
- With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.
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Content And Storage: resDNASEQ MDCK DNA Control• MDCK DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ MDCK DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• MDCK DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format: Kit
Host Cell: MDCK
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: Dry Ice
Includes: Assay Kit only
Content And Storage:
resDNASEQ MDCK DNA Control• MDCK DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ MDCK DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• MDCK DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format:
Kit
Host Cell:
MDCK
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
Dry Ice
Includes:
Assay Kit only
Content And Storage: resDNASEQ Pichia DNA Control• Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ Pichia DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format: Kit
Host Cell: Pichia
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: Dry Ice
Includes: Assay Kit only
Content And Storage:
resDNASEQ Pichia DNA Control• Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ Pichia DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format:
Kit
Host Cell:
Pichia
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
Dry Ice
Includes:
Assay Kit only
Content And Storage: resDNASEQ Pichia DNA Control• Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ Pichia DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format: Kit
Host Cell: Pichia
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: __
Includes: Assay Kit and Sample Preparation
Content And Storage:
resDNASEQ Pichia DNA Control• Pichia DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ Pichia DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• Pichia DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format:
Kit
Host Cell:
Pichia
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
__
Includes:
Assay Kit and Sample Preparation
Content And Storage: -20°C
Format: __
Host Cell: __
Product Line: __
No. of Reactions: __
Detection Method: __
PCR Method: __
Label or Dye: __
Shipping Condition: __
Includes: __
Content And Storage:
-20°C
Format:
__
Host Cell:
__
Product Line:
__
No. of Reactions:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Includes:
__