A55852
Applied Biosystems™ resDNASEQ™ dPCR E1A DNA Fragment Length Kit
Manufacturer: Applied Biosystems™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A55852-Each-of-1 | In Stock | ₹ 3,25,983.60 |
A55852 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,25,983.60
GST (18%): ₹ 58,677.048
Total Price: ₹ 3,84,660.648
Content And Storage
resDNASEQ E1A DNA Control• E1A DNA Control, 0.12 mL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E1A DNA dPCR Reagents• Absolute Q Digital PCR Master Mix, 5X, 2 x 360 μL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E1A DNA Assay Mixes (short, medium, long), 220 μL each, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Host Cell
HEK and other Cell Lines transformed by E1A
Product Type
Digital PCR Kit
No. of Reactions
100 reactions
PCR Method
Digital PCR
Product Line
resDNASEQ
Shipping Condition
Dry Ice
Related Products
Description
- The resDNASEQ dPCR E1A DNA Fragment Length Kit is an absolute quantitation PCR -based system that measures the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines, which are predominantly used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics
- Reliable and rapid, the resDNASEQ kit enables highly sensitive and specific quantitation of different fragment lengths of the E1A gene within the residual host-cell DNA
- This helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples with different sample matrices to purified final product—whether the sample contains high molecular weight or sheared DNA
- Features of the resDNASEQ dPCR E1A DNA Fragment Length Kit include: • Consistent performance across the expected range of DNA fragment sizes • Simple— streamlined workflow for ease-of-use with your digital PCR instrument • Fast—minimal hands-on time; results in ∼90 minutes when used with QuantStudio Absolute Q Digital PCR System • Reliable—consistent performance across expected range of DNA fragment sizes Integrated workflow solution The resDNASEQ dPCR E1A DNA Fragment Length Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing
- The resDNASEQ kit has been validated on the QuantStudio Absolute Q Digital PCR System
- The Absolute Q system has a high tolerance of common inhibitors in biopharmaceutical sample matrices, eliminating the need for nucleic acid extraction in most cases
- If nucleic acid extraction is required, we recommend use of the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow
- Easy to use With the resDNASEQ dPCR E1A DNA Fragment Length Kit, you perform dPCR analysis to quantitate the copy numbers of three different fragment size ranges in your test samples
- The resDNASEQ kit includes almost everything needed to carry out these steps, from master mix to TaqMan primer/probe mix to E1A DNA standards, as well as step-by-step instructions
- Consistent performance For accurate quantitation of fragments of three different lengths of E1A DNA, assay results must be unaffected by the size of non-specific DNA present in the test sample
- With the resDNASEQ system you get consistent assay performance across the three E1A fragment sizes.
SAFETY INFORMATION
- ShelfLife : 12 months
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Content And Storage: resDNASEQ E1A DNA Control• E1A DNA Control, 0.12 mL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E1A DNA dPCR Reagents• Absolute Q Digital PCR Master Mix, 5X, 2 x 360 μL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E1A DNA Assay Mixes (short, medium, long), 220 μL each, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Host Cell: HEK and other Cell Lines transformed by E1A
Product Type: Digital PCR Kit
No. of Reactions: 100 reactions
PCR Method: Digital PCR
Product Line: resDNASEQ
Shipping Condition: Dry Ice
Content And Storage:
resDNASEQ E1A DNA Control• E1A DNA Control, 0.12 mL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E1A DNA dPCR Reagents• Absolute Q Digital PCR Master Mix, 5X, 2 x 360 μL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E1A DNA Assay Mixes (short, medium, long), 220 μL each, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Host Cell:
HEK and other Cell Lines transformed by E1A
Product Type:
Digital PCR Kit
No. of Reactions:
100 reactions
PCR Method:
Digital PCR
Product Line:
resDNASEQ
Shipping Condition:
Dry Ice
Content And Storage: Sufficient for: 2,400 microplate assays or 250 test-tube assays• Dilution-Free Rapid Gold BCA Reagent A, 2 x 250 mL• Dilution-Free Rapid Gold BCA Reagent B, 10 mL• Dilution-Free BSA Protein Standards (0.2-10 mg/mL), 2 x 1 mL 8-channel tubestripStore in refrigerator (2°C to 8°C).
Host Cell: __
Product Type: Protein Quantitation Assay
No. of Reactions: __
PCR Method: __
Product Line: Pierce™ Dilution-Free™
Shipping Condition: Wet Ice
Content And Storage:
Sufficient for: 2,400 microplate assays or 250 test-tube assays• Dilution-Free Rapid Gold BCA Reagent A, 2 x 250 mL• Dilution-Free Rapid Gold BCA Reagent B, 10 mL• Dilution-Free BSA Protein Standards (0.2-10 mg/mL), 2 x 1 mL 8-channel tubestripStore in refrigerator (2°C to 8°C).
Host Cell:
__
Product Type:
Protein Quantitation Assay
No. of Reactions:
__
PCR Method:
__
Product Line:
Pierce™ Dilution-Free™
Shipping Condition:
Wet Ice
Content And Storage: Sufficient for: 1,200 microplate assays or 125 test-tube assays• Dilution-Free Rapid Gold BCA Reagent A, 250 mL• Dilution-Free Rapid Gold BCA Reagent B, 10 mL• Dilution-Free BSA Protein Standards (0.2-10 mg/mL), 2 x 1 mL 8-channel tubestripStore in refrigerator (2°C to 8°C).
Host Cell: __
Product Type: Protein Quantitation Assay
No. of Reactions: __
PCR Method: __
Product Line: Pierce™ Dilution-Free™
Shipping Condition: Wet Ice
Content And Storage:
Sufficient for: 1,200 microplate assays or 125 test-tube assays• Dilution-Free Rapid Gold BCA Reagent A, 250 mL• Dilution-Free Rapid Gold BCA Reagent B, 10 mL• Dilution-Free BSA Protein Standards (0.2-10 mg/mL), 2 x 1 mL 8-channel tubestripStore in refrigerator (2°C to 8°C).
Host Cell:
__
Product Type:
Protein Quantitation Assay
No. of Reactions:
__
PCR Method:
__
Product Line:
Pierce™ Dilution-Free™
Shipping Condition:
Wet Ice
Content And Storage: Sufficient for: 96 microplate assays or 10 test-tube assays• Dilution-Free Rapid Gold BCA Reagent A, 20 mL• Dilution-Free Rapid Gold BCA Reagent B, 1 mL• Dilution-Free BSA Protein Standards (0.2-10 mg/mL), 1 x 1 mL 8-channel tubestripStore in refrigerator (2°C to 8°C).
Host Cell: __
Product Type: Protein Quantitation Assay
No. of Reactions: __
PCR Method: __
Product Line: Pierce™ Dilution-Free™
Shipping Condition: Wet Ice
Content And Storage:
Sufficient for: 96 microplate assays or 10 test-tube assays• Dilution-Free Rapid Gold BCA Reagent A, 20 mL• Dilution-Free Rapid Gold BCA Reagent B, 1 mL• Dilution-Free BSA Protein Standards (0.2-10 mg/mL), 1 x 1 mL 8-channel tubestripStore in refrigerator (2°C to 8°C).
Host Cell:
__
Product Type:
Protein Quantitation Assay
No. of Reactions:
__
PCR Method:
__
Product Line:
Pierce™ Dilution-Free™
Shipping Condition:
Wet Ice