Thermo Scientific™ Luminaris Color Probe qPCR Master Mix

Description

• Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR
• The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer
• Only the template, primers and probe need to be added
• The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity
• dUTP and UDG are included in the mix for carry-over contamination control
• The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye
• The qPCR reaction mix containing both components is green
• The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates
• Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms
• Highlights Blue Master Mix and Yellow Sample Buffer for easy pipetting UDG in the master mix to prevent carry-over contamination Excellent qPCR performance Specificity — Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers Sensitivitity — single copy detection Wide linear range — accurate quantification across 9 orders of magnitude Reproducibility and convenience — ready-to-use 2X master mix Equal performance of blue and colorless master mixes Versions with premixed ROX for different real-time platforms Standard cycling protocols