4921658

Invitrogen™ DNA Polymerase I

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Description

DNA Polymerase

Exonuclease Activity

3' - 5', 5' - 3'

Hot Start

No

Polymerase

DNA Polymerase I

Shipping Condition

Wet Ice

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Description

  • Performance and quality testing Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined
  • Unit definition One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer
  • Unit reaction conditions 50mM potassium phosphate (pH 7.0), 6.7mM MgCl 2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [ 3H ]dTTP, and enzyme in 100μL for 30 min at 37°C
  • Also incorporates biotinylated nucleotides
  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs Source: purified from E
  • coli expressing the DNA Polymerase I gene on a plasmid Cloning, Restriction Enzyme Cloning Order Info Shipping Condition: Wet Ice

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