Thermo Scientific™ DreamTaq™ Buffers (10X)

Description

• Thermo Scientific DreamTaq Buffer (10X), available in colorless and green formats, is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase
• It contains both KCl and (NH 4 ) 2 SO 4 to provide high specificity of primer annealing at a wide range of MgCl 2 concentrations
• 10X DreamTaq buffer includes 20 mM MgCl 2 In addition, 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes
• The density reagent allows direct loading of PCR products on a gel
• The blue dye (migrates with 3–5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress
• The dyes have excitation peaks at 424 nm and 615 nm
• Notes For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq Buffer.