Thermo Scientific™ Phire Reaction Buffers

Description

• Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase
• This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR products on gel
• The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion
• Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel
• The dyes have excitation peaks at 424 nm and 615 nm
• The concentration of Mg 2+ in Phire Reaction Buffer is optimized to work well for most amplicons
• The buffer contains 1.5 mM MgCl 2 at final reaction concentration
• Separate tubes of MgCl 2 and DMSO are provided for further optimization
• Notes For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.