Thermo Scientific™ Phusion™ HF and GC Buffer Packs

Description

• The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10 -7 ) is lower than that in GC Buffer (9.5 x 10 -7 )
• Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification
• However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures
• For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers
• 5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel
• The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion
• Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free)) 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))