F518L
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F518L-Each-of-1 | In Stock | ₹ 4,383.25 |
F518L - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,383.25
GST (18%): ₹ 788.985
Total Price: ₹ 5,172.235
Content And Storage
• 5X Phusion HF Reaction Buffer (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase
Phusion High-Fidelity DNA Polymerase
Shipping Condition
Wet or Dry Ice
Starting Material
DNA
Color
Colorless
Description
Phusion™ HF Buffer Pack
Product Type
HF Buffer Pack
For Use With (Application)
Standard PCR, High-fidelity PCR
Volume
4 x 1.5 mL
Related Products
Description
- The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10 -7 ) is lower than that in GC Buffer (9.5 x 10 -7 )
- Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification
- However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures
- For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers
- 5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel
- The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion
- Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free)) 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
Compare Similar Items
Show Difference
Content And Storage: • 5X Phusion HF Reaction Buffer (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Shipping Condition: Wet or Dry Ice
Starting Material: DNA
Color: Colorless
Description: Phusion™ HF Buffer Pack
Product Type: HF Buffer Pack
For Use With (Application): Standard PCR, High-fidelity PCR
Volume: 4 x 1.5 mL
Content And Storage:
• 5X Phusion HF Reaction Buffer (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Shipping Condition:
Wet or Dry Ice
Starting Material:
DNA
Color:
Colorless
Description:
Phusion™ HF Buffer Pack
Product Type:
HF Buffer Pack
For Use With (Application):
Standard PCR, High-fidelity PCR
Volume:
4 x 1.5 mL
Content And Storage: • 5X Phusion GC Reaction Buffer (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Shipping Condition: Wet or Dry Ice
Starting Material: DNA
Color: Colorless
Description: Phusion™ GC Buffer Pack
Product Type: GC Buffer Pack
For Use With (Application): Standard PCR, High-fidelity PCR
Volume: 4 x 1.5 mL
Content And Storage:
• 5X Phusion GC Reaction Buffer (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Shipping Condition:
Wet or Dry Ice
Starting Material:
DNA
Color:
Colorless
Description:
Phusion™ GC Buffer Pack
Product Type:
GC Buffer Pack
For Use With (Application):
Standard PCR, High-fidelity PCR
Volume:
4 x 1.5 mL
Content And Storage: • 5X Phusion HF Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Shipping Condition: Wet or Dry Ice
Starting Material: DNA
Color: Colorless
Description: Detergent-free Phusion™ HF Buffer Pack
Product Type: Detergent-free FF Buffer Pack
For Use With (Application): Standard PCR, High-fidelity PCR
Volume: 4 x 1.5 mL
Content And Storage:
• 5X Phusion HF Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Shipping Condition:
Wet or Dry Ice
Starting Material:
DNA
Color:
Colorless
Description:
Detergent-free Phusion™ HF Buffer Pack
Product Type:
Detergent-free FF Buffer Pack
For Use With (Application):
Standard PCR, High-fidelity PCR
Volume:
4 x 1.5 mL
Content And Storage: • 5X Phusion GC Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Shipping Condition: Wet or Dry Ice
Starting Material: DNA
Color: Colorless
Description: Detergent-free Phusion™ GC Buffer Pack
Product Type: Detergent-free GC Buffer Pack
For Use With (Application): Standard PCR, High-fidelity PCR
Volume: 4 x 1.5 mL
Content And Storage:
• 5X Phusion GC Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Shipping Condition:
Wet or Dry Ice
Starting Material:
DNA
Color:
Colorless
Description:
Detergent-free Phusion™ GC Buffer Pack
Product Type:
Detergent-free GC Buffer Pack
For Use With (Application):
Standard PCR, High-fidelity PCR
Volume:
4 x 1.5 mL