F521L
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F521L-Each-of-1 | In Stock | ₹ 4,491.90 |
F521L - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,491.90
GST (18%): ₹ 808.542
Total Price: ₹ 5,300.442
Content And Storage
• 5X Phusion GC Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase
Phusion High-Fidelity DNA Polymerase
Shipping Condition
Wet or Dry Ice
Starting Material
DNA
Color
Colorless
Description
Detergent-free Phusion™ GC Buffer Pack
Product Type
Detergent-free GC Buffer Pack
For Use With (Application)
Standard PCR, High-fidelity PCR
Volume
4 x 1.5 mL
Related Products
Description
- The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10 -7 ) is lower than that in GC Buffer (9.5 x 10 -7 )
- Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification
- However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures
- For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers
- 5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel
- The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion
- Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free)) 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
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Content And Storage: • 5X Phusion GC Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Shipping Condition: Wet or Dry Ice
Starting Material: DNA
Color: Colorless
Description: Detergent-free Phusion™ GC Buffer Pack
Product Type: Detergent-free GC Buffer Pack
For Use With (Application): Standard PCR, High-fidelity PCR
Volume: 4 x 1.5 mL
Content And Storage:
• 5X Phusion GC Reaction Buffer, Detergent-free (4 x 1.5 mL)• 50 mM MgCl2(4 x 1.5 mL)• DMSO (0.5 mL)Store at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Shipping Condition:
Wet or Dry Ice
Starting Material:
DNA
Color:
Colorless
Description:
Detergent-free Phusion™ GC Buffer Pack
Product Type:
Detergent-free GC Buffer Pack
For Use With (Application):
Standard PCR, High-fidelity PCR
Volume:
4 x 1.5 mL
Content And Storage: • 5X Phire Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase: Phire Hot Start II DNA Polymerase
Shipping Condition: Wet or Dry Ice
Starting Material: __
Color: __
Description: Colorless
Product Type: Phire Reaction Buffer
For Use With (Application): Standard PCR
Volume: 6 mL
Content And Storage:
• 5X Phire Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase:
Phire Hot Start II DNA Polymerase
Shipping Condition:
Wet or Dry Ice
Starting Material:
__
Color:
__
Description:
Colorless
Product Type:
Phire Reaction Buffer
For Use With (Application):
Standard PCR
Volume:
6 mL
Content And Storage: __
Polymerase: __
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