F525L
Thermo Scientific™ Phire Reaction Buffers
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F525L-Each-of-1 | In Stock | ₹ 3,935.76 |
F525L - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,935.76
GST (18%): ₹ 708.437
Total Price: ₹ 4,644.197
Content And Storage
• 5X Phire Reaction Buffer, detergent-free, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• 100% DMSO, 0.5 mLStore at -20°C.
Polymerase
Phire Hot Start II DNA Polymerase
Quantity
4 x 1.5 mL
For Use With (Application)
Standard PCR
Volume
6 mL
Description
Detergent-free
Product Type
Phire Reaction Buffer, detergent-free
Shipping Condition
Wet or Dry Ice
Concentration
5X
Related Products
Description
- Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase
- This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR products on gel
- The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion
- Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel
- The dyes have excitation peaks at 424 nm and 615 nm
- The concentration of Mg 2+ in Phire Reaction Buffer is optimized to work well for most amplicons
- The buffer contains 1.5 mM MgCl 2 at final reaction concentration
- Separate tubes of MgCl 2 and DMSO are provided for further optimization
- Notes For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.
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Content And Storage: • 5X Phire Reaction Buffer, detergent-free, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• 100% DMSO, 0.5 mLStore at -20°C.
Polymerase: Phire Hot Start II DNA Polymerase
Quantity: 4 x 1.5 mL
For Use With (Application): Standard PCR
Volume: 6 mL
Description: Detergent-free
Product Type: Phire Reaction Buffer, detergent-free
Shipping Condition: Wet or Dry Ice
Concentration: 5X
Content And Storage:
• 5X Phire Reaction Buffer, detergent-free, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• 100% DMSO, 0.5 mLStore at -20°C.
Polymerase:
Phire Hot Start II DNA Polymerase
Quantity:
4 x 1.5 mL
For Use With (Application):
Standard PCR
Volume:
6 mL
Description:
Detergent-free
Product Type:
Phire Reaction Buffer, detergent-free
Shipping Condition:
Wet or Dry Ice
Concentration:
5X
Content And Storage: __
Polymerase: __
Quantity: __
For Use With (Application): __
Volume: __
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Content And Storage: • 5X Phire Green Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase: Phire Hot Start II DNA Polymerase
Quantity: 4 x 1.5 mL
For Use With (Application): Hot-start PCR
Volume: __
Description: Green
Product Type: Phire Green Reaction Buffer
Shipping Condition: Wet or Dry Ice
Concentration: 5X
Content And Storage:
• 5X Phire Green Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase:
Phire Hot Start II DNA Polymerase
Quantity:
4 x 1.5 mL
For Use With (Application):
Hot-start PCR
Volume:
__
Description:
Green
Product Type:
Phire Green Reaction Buffer
Shipping Condition:
Wet or Dry Ice
Concentration:
5X