F527L
Thermo Scientific™ Phire Reaction Buffers
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F527L-Each-of-1 | In Stock | ₹ 3,691.91 |
F527L - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,691.91
GST (18%): ₹ 664.544
Total Price: ₹ 4,356.454
Content And Storage
• 5X Phire Green Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase
Phire Hot Start II DNA Polymerase
Quantity
4 x 1.5 mL
For Use With (Application)
Hot-start PCR
Description
Green
Product Type
Phire Green Reaction Buffer
Shipping Condition
Wet or Dry Ice
Concentration
5X
Related Products
Description
- Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase
- This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR products on gel
- The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion
- Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel
- The dyes have excitation peaks at 424 nm and 615 nm
- The concentration of Mg 2+ in Phire Reaction Buffer is optimized to work well for most amplicons
- The buffer contains 1.5 mM MgCl 2 at final reaction concentration
- Separate tubes of MgCl 2 and DMSO are provided for further optimization
- Notes For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.
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Content And Storage: • 5X Phire Green Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase: Phire Hot Start II DNA Polymerase
Quantity: 4 x 1.5 mL
For Use With (Application): Hot-start PCR
Description: Green
Product Type: Phire Green Reaction Buffer
Shipping Condition: Wet or Dry Ice
Concentration: 5X
Content And Storage:
• 5X Phire Green Reaction Buffer, 4 x 1.5 mL• 50 mM MgCl2, 2 x 1.5 mL• DMSO, 0.5 mLStore at -20°C.
Polymerase:
Phire Hot Start II DNA Polymerase
Quantity:
4 x 1.5 mL
For Use With (Application):
Hot-start PCR
Description:
Green
Product Type:
Phire Green Reaction Buffer
Shipping Condition:
Wet or Dry Ice
Concentration:
5X
Content And Storage: • Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Quantity: 500 U
For Use With (Application): Standard PCR, High-fidelity PCR
Description: __
Product Type: High-Fidelity DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Content And Storage:
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Quantity:
500 U
For Use With (Application):
Standard PCR, High-fidelity PCR
Description:
__
Product Type:
High-Fidelity DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Content And Storage: • Phusion High-Fidelity DNA Polymerase (4 x 500 U at 2 U/μL)• 10 mM dNTP Mix (2 x 1 mL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionStore at –5°C to –30°C.
Polymerase: Phusion High Fidelity DNA Polymerase
Quantity: __
For Use With (Application): Standard PCR, High-fidelity PCR
Description: __
Product Type: High-Fidelity DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Content And Storage:
• Phusion High-Fidelity DNA Polymerase (4 x 500 U at 2 U/μL)• 10 mM dNTP Mix (2 x 1 mL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionStore at –5°C to –30°C.
Polymerase:
Phusion High Fidelity DNA Polymerase
Quantity:
__
For Use With (Application):
Standard PCR, High-fidelity PCR
Description:
__
Product Type:
High-Fidelity DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Content And Storage: • Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Quantity: 100 U
For Use With (Application): Standard PCR, High-fidelity PCR
Description: __
Product Type: High-Fidelity DNA Polymerase
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Content And Storage:
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Quantity:
100 U
For Use With (Application):
Standard PCR, High-fidelity PCR
Description:
__
Product Type:
High-Fidelity DNA Polymerase
Shipping Condition:
Dry Ice
Concentration:
2 U/μL