Invitrogen™ SuperScript™ IV Reverse Transcriptase

Description

• SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions
• It is significantly improved over SuperScript III RT in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of that enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity
• SuperScript IV RT is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples that cause other currently available RTs to perform inefficiently
• SuperScript IV RT offers advantages at standard as well as elevated temperatures over cloned AMV and ThermoScript RT, including higher sensitivity, inhibitor tolerance, and ability to work with degraded RNA
• Features of SuperScript IV Reverse Transcriptase include: • Significantly improved resistance to inhibitors that can interfere with cDNA synthesis • Robust and specific cDNA synthesis in a wide range of sample types • Faster reverse transcriptase reaction reduces incubation time from >50 minutes to 10 minutes • Better processivity when compared to SuperScript III RT SuperScript IV RT is the top choice for all RT-PCR and qRT-PCR applications in the SuperScript product family when reproducibility and reliability is the primary concern
• It is also our top performing RT when inhibitors in the RNA sample may interfere with cDNA synthesis, leading to biases in gene expression studies
• Source Purified from E
• coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance and to reduce RNase H activity Unit definition One unit of SuperScript IV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT) 12-18 as a template/primer
• Unit reaction conditions 50 mM Tris-HCl (pH 8.3), 4 mM MgCl 2 , 10 mM DTT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/mL [ 3 H]-dTTP, 0.4 mM poly(A) oligo (dT) 12-18 and enzyme in 20 μl for 10 min at 37°C.