4926554
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
Manufacturer: Fischer Scientific
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Format
Stand-alone Enzyme
Reaction Speed
50 min.
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Reduced
Concentration
200 U/μL
Reaction Format
Separate components
Size (Final Product)
Up to 12.3 kb
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript II
Shipping Condition
Wet Ice
Final Product Type
First-Strand cDNA
Reagent Type
Reverse Transcription
Starting Material
RNA
Related Products
Description
- Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT
- Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA
- SuperScript reverse transcriptases are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E
- coli containing the modified pol gene of Moloney Murine Leukemia Virus
- Features of SuperScript II Reverse Transcriptase include: • High cDNA yields and more full-length product • Generation of cDNA up to 12.3 kb • First-strand cDNA synthesis at higher temperatures than conventional MMLV RT Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase (Cat
- No
- 18090010), features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity
- Order Info Shipping Condition: Wet ice
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Format: Stand-alone Enzyme
Reaction Speed: 50 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript II
Shipping Condition: Wet Ice
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Format:
Stand-alone Enzyme
Reaction Speed:
50 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript II
Shipping Condition:
Wet Ice
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format: Stand-alone Enzyme
Reaction Speed: 30 to 50 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Format:
Stand-alone Enzyme
Reaction Speed:
30 to 50 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format: Kit
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
Concentration: __
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Format:
Kit
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
Concentration:
__
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
Concentration: __
Reaction Format: __
Size (Final Product): __
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Final Product Type: __
Reagent Type: __
Starting Material: __
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
Concentration:
__
Reaction Format:
__
Size (Final Product):
__
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Final Product Type:
__
Reagent Type:
__
Starting Material:
__