Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Description

• Thermo Scientific DreamTaq Hot Start PCR Master Mix, available in colorless and green formats, is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq Buffer, magnesium, and dNTPs
• The master mix allows easy reaction setup and fewer pipetting steps, with only primers, template, and water needing to be added
• The DreamTaq Hot Start Green PCR Master Mix includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel
• DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases
• DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step
• With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases
• DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity through: Better reaction outcomes Higher yield of target amplicons from low template amounts Increased reaction specificity due to reliable hot-start technology Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb Enhanced convenience Room temperature reaction setup Minimized optimization of Mg 2+ concentration and of primer annealing temperatures due to optimized reaction buffer Simplified workflow and minimized pipetting steps with 2X master mix format Direct loading of PCR products on a gel with green format Applications Generation of PCR products for TA cloning Routine PCR Colony PCR Genotyping RT-PCR