Thermo Scientific™ PCR Master Mix (2X)

Description

• Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers
• This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up
• The mix is optimized for efficient and reproducible PCR
• Highlights Convenient, ready-to-use mix Thermostable—half life is more than 40 min
• at 95°C Generates PCR products with 3'-dA overhangs Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides) Applications Routine PCR amplification of DNA fragments up to 5 kb High throughput PCR DNA labeling Note The error rate of Taq DNA Polymerase in PCR is 2.2 x 10 -5 errors per nt per cycle, as determined by a modified method described in
• Accordingly, the accuracy of PCR is 4.5 x 10 4
• Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.