Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Description

• Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix
• It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications and reproducible cDNA synthesis at elevated temperatures (50–65°C) within 30 minutes
• The master mix contains Maxima H Minus Reverse Transcriptase (RT) and RiboLock RNase Inhibitor
• Maxima H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution
• The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity
• The recombinant RiboLock RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55°C
• The master mix also contains reaction buffer, dNTPs, oligo (dT) 18 , and random hexamer primers
• A separate tube of No-RT control mix is also provided for convienient RT negative control
• Both kits are capable of reproducible cDNA synthesis at elevated temperatures (50°C–65°C)
• The synthesis reaction is typically complete in 15–30 minutes
• The included double-strand specific DNase in the Maxima H Minus cDNA Synthesis Master Mix with dsDNase allows removal of genomic DNA from RNA samples in two minutes without affecting the quality or quantity of RNA
• This dsDNase is also available separately (Cat
• No
• EN0771)
• Features of Maxima H Minus cDNA Synthesis Master Mix include: • One-tube master mix helps reduce pipetting steps and enhanced consistency in RT-qPCR results • Increased RT efficiency across a wide range of input RNA amounts and gene targets • High thermostability to allow RT reactions at 50–65°C temperature range • Integrated step of genomic DNA removal from RNA samples (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only) Additional information about reaction components • The No RT control mix contains all the components in the Maxima H Minus cDNA Synthesis Master Mix except RT
• The presence of a PCR product in the No RT control reaction indicates that the reaction is contaminated with DNA
• To further enhance genomic DNA elimination efficiency, template RNA incubation with dsDNase (Cat
• No
• EN0771) is strongly recommended
• • Nuclease-free water is provided for reaction setup and dilution of sample DNA
• The absence of exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests
• • To further enhance genomic DNA elimination efficiency, template RNA incubation with the included dsDNase is strongly recommended
• (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only) • The dsDNase is used for rapid and safe removal of contaminating genomic DNA from RNA samples
• dsDNase is easily inactivated by moderate heat treatment (55°C)
• It allows for a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure
• (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only)