Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

Description

• The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA
• Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR
• Features of the SuperScript IV CellsDirect Synthesis Kit • Compatible with a wide range of mammalian cell types grown under different treatment conditions • Single-tube format minimizes reagent loss, sample loss, and handling time • Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts • High-quality cDNA generated for use in a variety of applications, including PCR and qPCR • Simple protocol takes approximately 37 minutes • Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers • Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA Features of the SuperScript IV Reverse Transcriptase included in this kit • Reduced RNase H activity • High thermal stability • High yields of cDNA in the first-strand synthesis reaction for greater sensitivity and enhanced detection of rare transcripts • Superior tolerance to various RT inhibitors SuperScript IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications
• It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions
• It is significantly improved over SuperScript III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity
• How it works In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material
• Using the SuperScript IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss
• DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis
• This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.