4926559
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
Manufacturer: Fischer Scientific
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Detection Method
qPCR
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Kit
Reaction Speed
60 min.
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Reduced
For Use With (Application)
Real Time PCR (qPCR)
Reaction Format
Master Mix
Size (Final Product)
Up to 10 kb
Related Products
Description
- The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications
- The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes
- The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis
- The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR
- Notes • For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat
- No
- 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat
- No
- 11766050)
- • This kit is included with EXPRESS Two-Step RT–qPCR kits
- Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions
- Order Info Shipping Condition: Dry ice
Compare Similar Items
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Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Detection Method: __
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Final Product Type: __
Reagent Type: __
Starting Material: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Reaction Format: __
Size (Final Product): __
Detection Method:
__
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Final Product Type:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
Reaction Format:
__
Size (Final Product):
__
Detection Method: __
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Final Product Type: __
Reagent Type: __
Starting Material: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Reaction Format: __
Size (Final Product): __
Detection Method:
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GC-Rich PCR Performance:
__
Technique:
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Reverse Transcriptase:
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Shipping Condition:
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Final Product Type:
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Reagent Type:
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Starting Material:
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Format:
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Reaction Speed:
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Optimal Reaction Temperature:
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Ribonuclease H Activity:
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For Use With (Application):
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Reaction Format:
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Size (Final Product):
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Detection Method: __
GC-Rich PCR Performance: Low
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Final Product Type: __
Reagent Type: __
Starting Material: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): Hot-start PCR
Reaction Format: __
Size (Final Product): __
Detection Method:
__
GC-Rich PCR Performance:
Low
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Final Product Type:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
Hot-start PCR
Reaction Format:
__
Size (Final Product):
__