Invitrogen™ SuperScript™ VILO™ Master Mix

Description

• SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications
• The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations
• The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays
• Features of SuperScript VILO Master Mix include: • Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction • Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination • RT is engineered with reduced RNase H activity and increased thermal stability For superior cDNA synthesis performance in RT-qPCR applications SuperScript IV VILO Master Mix (Cat
• No
• 11756050) elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer
• The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.