11-754-050
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-754-050-Each-of-1 | In Stock | ₹ 59,378.64 |
11-754-050 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 59,378.64
GST (18%): ₹ 10,688.155
Total Price: ₹ 70,066.795
Content And Storage
• 5X VILO Reaction Mix, 200 μL• 10X SuperScript III Enzyme Blend, 100 μLStore at –5 to –30°C.
Format
Kit
Reaction Speed
60 min.
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Reduced
For Use With (Application)
Real Time PCR (qPCR)
No. of Reactions
50 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Detection Method
qPCR
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Master Mix
Size (Final Product)
Up to 10 kb
Related Products
Description
- The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications
- The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes
- The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis
- The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR
- Notes • For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat
- No
- 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat
- No
- 11766050)
- • This kit is included with EXPRESS Two-Step RT–qPCR kits
- Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions
- Order Info Shipping Condition: Dry ice
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Content And Storage: • 5X VILO Reaction Mix, 200 μL• 10X SuperScript III Enzyme Blend, 100 μLStore at –5 to –30°C.
Format: Kit
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Content And Storage:
• 5X VILO Reaction Mix, 200 μL• 10X SuperScript III Enzyme Blend, 100 μLStore at –5 to –30°C.
Format:
Kit
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Content And Storage: • 5X VILO Reaction Mix, 1,000 μL• 10X SuperScript III Enzyme Blend, 500 μLStore at –5 to –30°C.
Format: Kit
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 250 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Content And Storage:
• 5X VILO Reaction Mix, 1,000 μL• 10X SuperScript III Enzyme Blend, 500 μLStore at –5 to –30°C.
Format:
Kit
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
250 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Content And Storage: • SuperScript VILO Master Mix (200 μL)Store at –5 to –30°C.
Format: Master Mix
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Content And Storage:
• SuperScript VILO Master Mix (200 μL)Store at –5 to –30°C.
Format:
Master Mix
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Content And Storage: • SuperScript VILO Master Mix (1 mL)Store at –5 to –30°C.
Format: Master Mix
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 250 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Content And Storage:
• SuperScript VILO Master Mix (1 mL)Store at –5 to –30°C.
Format:
Master Mix
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
250 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb