11-755-250
Invitrogen™ SuperScript™ VILO™ Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-755-250-Each-of-1 | In Stock | ₹ 2,61,813.60 |
11-755-250 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,61,813.60
GST (18%): ₹ 47,126.448
Total Price: ₹ 3,08,940.048
Content And Storage
• SuperScript VILO Master Mix (1 mL)Store at –5 to –30°C.
Format
Master Mix
Reaction Speed
60 min.
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Reduced
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
Reaction Format
Master Mix
Size (Final Product)
Up to 10 kb
Detection Method
qPCR
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
Concentration
5X
No. of Reactions
250 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Related Products
Description
- SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications
- The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations
- The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays
- Features of SuperScript VILO Master Mix include: • Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction • Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination • RT is engineered with reduced RNase H activity and increased thermal stability For superior cDNA synthesis performance in RT-qPCR applications SuperScript IV VILO Master Mix (Cat
- No
- 11756050) elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer
- The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
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Content And Storage: • SuperScript VILO Master Mix (1 mL)Store at –5 to –30°C.
Format: Master Mix
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Concentration: 5X
No. of Reactions: 250 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript VILO Master Mix (1 mL)Store at –5 to –30°C.
Format:
Master Mix
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Concentration:
5X
No. of Reactions:
250 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: • SuperScript VILO Master Mix (2 mL)Store at –5 to –30°C.
Format: Master Mix
Reaction Speed: 60 min.
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Concentration: 5X
No. of Reactions: 500 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript VILO Master Mix (2 mL)Store at –5 to –30°C.
Format:
Master Mix
Reaction Speed:
60 min.
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Concentration:
5X
No. of Reactions:
500 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: • SuperScript IV VILO Master Mix (200 μL)• SuperScript IV VILO Master Mix 'No RT' Control (200 μL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format: Master Mix
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Concentration: 5X
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript IV VILO Master Mix (200 μL)• SuperScript IV VILO Master Mix 'No RT' Control (200 μL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format:
Master Mix
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Concentration:
5X
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: • SuperScript IV VILO Master Mix (2 x 1 mL)• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format: Master Mix
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Concentration: 5X
No. of Reactions: 500 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript IV VILO Master Mix (2 x 1 mL)• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format:
Master Mix
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Concentration:
5X
No. of Reactions:
500 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA