Applied Biosystems™ TaqMan™ Genotyping Master Mix

Description

• Distinct clusters and high call rates for unambiguous allelic discrimination Validated with TaqMan™ SNP Genotyping Assays Excellent pre- and post-PCR stability for high throughput setup and analysis Reaction size is based on a 50μL reaction volume May be used for a variety of genotyping applications, including: Candidate gene studies Drug-target validation Disease association studies Population genetics Linkage mapping Agricultural applications Exceptional Performance from an Optimized Mix (2X) Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target
• TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution
• Key features include: AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup Optimized mix components provide excellent specificity for discrimination between alleles Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments Single thermal cycling condition for consistent results with TaqMan™ Assays Validated with TaqMan™ Genotyping Assays and Instruments: TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems High-Throughput Setup and Analysis: The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR This provides the flexibility required to run long experiments, unattended overnight or over a weekend Reliable Discrimination with Challenging Targets Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets Setting the Industry Standard for Quality: Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site Order Info Guaranteed minimum shelf life is 60 days
• Shipping Conditions: Wet Ice