MilliporeSigma™ Novagen™ One Step RT-PCR Master Mix Kit

Description

• Robust one-step, one-enzyme master mix system for easy reaction assembly Eliminates risk of cross contamination associated with two-step RT-PCR protocols High-temperature (60° C) for reverse transcription enhances read-through of RNA secondary structure Optimized buffer conditions and antibody-mediated hot start for increased sensitivity Rapid enzyme activation step (30 s) avoids damage of template RNA Components: 2 x 625μL 2X One Step RT-PCR Master Mix 1 x 200μL 50 mM Mn(OAc)2 1 x 1.1mL RNase Free Water 1 x 50μL Primer F (10 pmol/μL, 5′-TCC ACC ACC CTG TTG CTG TA-3′) 1 x 50μL Primer R (10 pmol/μL, 5′-ACC ACA GTC CAT GCC ATC AC-3′) 1 x 50μL Positive Control RNA (5 x 108 copies/mL)