Applied Biosystems™ TrueMark™ Comprehensive Microbiota Control

Description

• The TrueMark Comprehensive Microbiota Control contains a linearized multi-target plasmid pool with microbial target sequences commonly used on Custom OpenArray panels
• The control has targets relevant to many microbial profiling applications, including respiratory tract, urinary tract, vaginal microbiota, and antibiotic resistance research
• The control can be included as a positive control for panel-specific amplification
• It is available in two concentrations: 1 x 10 5 and 5 x 10 7 copies/μL
• The higher concentration can be used to determine parameters necessary for analytical validation (e.g., limit of detection and Ct cut-off values)
• The plasmid also contains target sequences for TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control, TaqMan Universal Extraction Control Organism (B
• atrophaeus), and the human RNase P RPPH1 gene
• During real-time PCR, the control can be used as a stand-alone sample to verify assay performance and to help with troubleshooting
• Note: This product will be updated on an annual basis to ensure coverage of the most popular microbial targets, and performance will be measured to ensure consistency between versions
• Version History: Version 1 of this product was first available in April 2021 and contained 471 microbial assay targets
• Version 2 became available in October 2021 and contains an additional 83 microbial assay targets
• Version 3 became avalible in March 2023 and contains an additional 139  microbial and antibiotic resistant targets.