Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Description

• Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA
• It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications
• Features of Platinum Direct PCR Universal Master Mix include: Direct DNA amplification from various samples Universal primer annealing at 60°C Optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets Superior specificity, sensitivity, and yields Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples
• The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments
• Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material
• Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step
• This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature
• Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules
• Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair
• Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.