Applied Biosystems™ Absolute Q™ dPCR SARS-CoV-2 Wastewater Surveillance Kit

Description

• The Applied Biosystems Absolute Q dPCR SARS-CoV-2 Wastewater Surveillance Kit enables fast, sensitive, absolute quantification of SARS-CoV-2 from isolated wastewater nucleic acid using the QuantStudio Absolute Q Digital PCR System
• Features of the Absolute Q dPCR SARS-CoV-2 wastewater surveillance kit include: • Ease of use —simple qPCR-like workflow with just a few pipetting steps and minimal hands-on time • Fast —absolute quantification of targets of interest in less than two hours, including reaction preparation and dPCR data collection • Consistency —powered by microfluidic array plate (MAP) technology and TaqMan chemistry for exceptional data consistency The Absolute Q SARS-CoV-2 Wastewater Surveillance Kit contains both a triplex digital PCR (dPCR) assay and Absolute Q 1-Step RT-dPCR Master Mix (Cat
• No
• A55146)
• The high-resolution multiplex assay targets two SARS-CoV-2 N gene (nucleoplasmid protein) regions because the gene is the more stable and less susceptible to mutations
• The assay was designed by our experts with experience in rapidly responding to the SARS-CoV-2 outbreak, who helped ensure SARS-CoV-2 specificity and followed CDC guidance
• In accordance with CDC guidelines for SARS-CoV-2 wastewater surveillance, an assay targeting pepper mild mottle virus (PMMoV) is also part of the single tube triplex assay to enable fecal normalization
• The assay enables all three targets to be detected in a single well
• With the QuantStudio Absolute Q dPCR System, the red channel is free to enable further multiplexing with your own target of interest by combining the assay kit with an appropriate dye-labeled assay
• The wastewater surveillance kit offers enough multiplex assay and master mix for 200 sample reactions and is for use with the QuantStudio Absolute Q MAP16 Plate
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