A65424
Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A65424-Each-of-1 | In Stock | ₹ 94,458.24 |
A65424 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 94,458.24
GST (18%): ₹ 17,002.483
Total Price: ₹ 1,11,460.723
Content And Storage
• 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)• 10X Lysis Buffer (96 μL)• Nuclease-free water (1.25 mL)Store at -15 to -25°C.
GC-Rich PCR Performance
High
Technique
Template Switching Reverse Transcription
Final Product Type
First-Strand cDNA
Reagent Type
Reverse Transcription
Format
Master Mix
Reaction Speed
30 min.
Concentration
4X
No. of Reactions
96 Reactions
Starting Material
Cells or RNA
Related Products
Description
- Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions
- It contains SuperScript IV Reverse Transcriptase, RNase Inhibitor, and all other reverse transcription reaction components in a convenient premixed format
- Only RNA template, RT primer, and template switching oligo (TSO) of choice need to be added
- Features of SuperScript IV Template-Switching RT Master Mix include: • Formulated for high quality full-length cDNA synthesis • Includes proprietary enhancer to maximize template switching efficiency • Exceptional sensitivity for easy detection of low abundance targets (as low as single cell or 2 pg total RNA) • Cell lysis buffer included for direct cDNA synthesis without RNA purification Procedure overview To maximize template switching efficiency, SuperScript IV Template-Switching RT Master Mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (TdT) activity of SuperScript IV Reverse Transcriptase
- When the reverse transcriptase reaches the 5’ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling binding of the template switching oligo and incorporation of known sequence at the 3’ end of the resulting cDNA
- This cDNA can be used for amplification by PCR, gene expression analysis by real-time PCR, or as a template for 5’RACE, RNA-seq, or second strand cDNA synthesis.This master mix is compatible with a wide input range of total RNA (from 2 pg to 4 µg) and is supplied with lysis buffer to enable direct cDNA synthesis from mammalian cells (1–1,000 cells per sample)
- Related products SuperScript IV Template-Switching RT Master Mix is compatible with various RT primers and self-designed template switching oligos
- We offer Template Switching Oligo (Cat
- No
- A65427) and Capturing Oligo (dT) Primer (A65429), both containing the same adaptor sequence complementary to Preamplification Primer (A65428)
- Alternatively, a full set of reagents for template switching cDNA synthesis and preamplification is available in the SuperScript IV Single Cell/Low-Input cDNA PreAmp Kit (11752048).
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Content And Storage: • 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)• 10X Lysis Buffer (96 μL)• Nuclease-free water (1.25 mL)Store at -15 to -25°C.
GC-Rich PCR Performance: High
Technique: Template Switching Reverse Transcription
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Format: Master Mix
Reaction Speed: 30 min.
Concentration: 4X
No. of Reactions: 96 Reactions
Starting Material: Cells or RNA
Content And Storage:
• 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)• 10X Lysis Buffer (96 μL)• Nuclease-free water (1.25 mL)Store at -15 to -25°C.
GC-Rich PCR Performance:
High
Technique:
Template Switching Reverse Transcription
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Format:
Master Mix
Reaction Speed:
30 min.
Concentration:
4X
No. of Reactions:
96 Reactions
Starting Material:
Cells or RNA
Content And Storage: Five boxes, each containing:• 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)• 10X Lysis Buffer (96 μL)• Nuclease-free water (1.25 mL)Store at -15 to -25°C.
GC-Rich PCR Performance: High
Technique: Template Switching Reverse Transcription
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Format: Master Mix
Reaction Speed: 30 min.
Concentration: 4X
No. of Reactions: 5 x 96 Reactions
Starting Material: Cells or RNA
Content And Storage:
Five boxes, each containing:• 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)• 10X Lysis Buffer (96 μL)• Nuclease-free water (1.25 mL)Store at -15 to -25°C.
GC-Rich PCR Performance:
High
Technique:
Template Switching Reverse Transcription
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Format:
Master Mix
Reaction Speed:
30 min.
Concentration:
4X
No. of Reactions:
5 x 96 Reactions
Starting Material:
Cells or RNA
Content And Storage: __
GC-Rich PCR Performance: __
Technique: __
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Reaction Speed: __
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GC-Rich PCR Performance:
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Reagent Type:
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Format:
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Reaction Speed:
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Concentration:
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Starting Material:
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Content And Storage: Template Switching Oligo, 48 μLStore at -25°C to -15°C.
GC-Rich PCR Performance: __
Technique: __
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Reagent Type: __
Format: __
Reaction Speed: __
Concentration: 20X
No. of Reactions: __
Starting Material: __
Content And Storage:
Template Switching Oligo, 48 μLStore at -25°C to -15°C.
GC-Rich PCR Performance:
__
Technique:
__
Final Product Type:
__
Reagent Type:
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Format:
__
Reaction Speed:
__
Concentration:
20X
No. of Reactions:
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Starting Material:
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