Applied Biosystems™ CLA IdentiFiler™ Plus PCR Amplification Kit

Description

• The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines
• It includes the same primers and allelic ladder as the widely used AmpFLSTR Identifiler Plus kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance with mixtures, and the ability to overcome high levels of PCR inhibition
• The kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data
• Features of the CLA IdentiFiler Plus PCR Amplification Kit include: • 5-dye, 16-locus STR kit compatible with all cell line databasing loci standards • Improved version of legacy AmpFLSTR Identifiler Plus kit • Size standard included for ease of purchase • Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzers Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA)
• In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check
• For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.