A65909
Applied Biosystems™ CLA GlobalFiler™ PCR Amplification Kit
Manufacturer: Applied Biosystems™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A65909-Each-of-1 | In Stock | ₹ 5,62,129.20 |
A65909 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,62,129.20
GST (18%): ₹ 1,01,183.256
Total Price: ₹ 6,63,312.456
Content And Storage
• GlobalFiler PCR Amplification Kit• GS Liz600 Size Standard
Form
Liquid
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold™ DNA Polymerase
Technique
STR (Short Tandem Repeats) Analysis
Kit Type
Multicomponent
Quantity
200 x 25 μL Reactions
Target Organism Class
Human cells
Purification Target
Genomic DNA
Sample Type
Genomic DNA
Detection Method
Gel Electrophoresis
Format
Kit
PCR Method
End-point PCR
Reaction Speed
60 min.
For Use With (Equipment)
SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers
Label or Dye
LIZ, FAM, NED, PET, VIC
Species
Human
For Use With (Application)
Genotyping, Cell Analysis
Reaction Format
Master Mix
Starting Material
Cell Line Culture
Related Products
Description
- The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines
- GlobalFiler PCR amplification kits are 6-dye, 24-locus short tandem repeat (STR) analysis kits that combine maximum compatibility with global databasing loci standards with dramatically reduced amplification time and superior discrimination power, enabling labs with improved overall efficiency
- GlobalFiler kits are backed by best-in-class global training, service, and support
- This kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data
- Features of the CLA GlobalFiler PCR Amplification Kit include: 6-dye, 24-locus STR analysis kit compatible with all cell line databasing loci standards Maximum compatibility with global databasing loci standards Reduced amplification time and superior discrimination power Size standard included for ease of purchase Compatible with SeqStudio, 3730/xL, 3500/xL and SeqStudio Flex (8- and 24-capillary) genetic analyzers Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA)
- In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check
- For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.
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Content And Storage: • GlobalFiler PCR Amplification Kit• GS Liz600 Size Standard
Form: Liquid
GC-Rich PCR Performance: High
Polymerase: AmpliTaq Gold™ DNA Polymerase
Technique: STR (Short Tandem Repeats) Analysis
Kit Type: Multicomponent
Quantity: 200 x 25 μL Reactions
Target Organism Class: Human cells
Purification Target: Genomic DNA
Sample Type: Genomic DNA
Detection Method: Gel Electrophoresis
Format: Kit
PCR Method: End-point PCR
Reaction Speed: 60 min.
For Use With (Equipment): SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers
Label or Dye: LIZ, FAM, NED, PET, VIC
Species: Human
For Use With (Application): Genotyping, Cell Analysis
Reaction Format: Master Mix
Starting Material: Cell Line Culture
Content And Storage:
• GlobalFiler PCR Amplification Kit• GS Liz600 Size Standard
Form:
Liquid
GC-Rich PCR Performance:
High
Polymerase:
AmpliTaq Gold™ DNA Polymerase
Technique:
STR (Short Tandem Repeats) Analysis
Kit Type:
Multicomponent
Quantity:
200 x 25 μL Reactions
Target Organism Class:
Human cells
Purification Target:
Genomic DNA
Sample Type:
Genomic DNA
Detection Method:
Gel Electrophoresis
Format:
Kit
PCR Method:
End-point PCR
Reaction Speed:
60 min.
For Use With (Equipment):
SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers
Label or Dye:
LIZ, FAM, NED, PET, VIC
Species:
Human
For Use With (Application):
Genotyping, Cell Analysis
Reaction Format:
Master Mix
Starting Material:
Cell Line Culture
Content And Storage: __
Form: __
GC-Rich PCR Performance: __
Polymerase: __
Technique: __
Kit Type: __
Quantity: __
Target Organism Class: __
Purification Target: __
Sample Type: __
Detection Method: __
Format: __
PCR Method: __
Reaction Speed: __
For Use With (Equipment): __
Label or Dye: __
Species: __
For Use With (Application): __
Reaction Format: __
Starting Material: __
Content And Storage:
__
Form:
__
GC-Rich PCR Performance:
__
Polymerase:
__
Technique:
__
Kit Type:
__
Quantity:
__
Target Organism Class:
__
Purification Target:
__
Sample Type:
__
Detection Method:
__
Format:
__
PCR Method:
__
Reaction Speed:
__
For Use With (Equipment):
__
Label or Dye:
__
Species:
__
For Use With (Application):
__
Reaction Format:
__
Starting Material:
__
Content And Storage: Sufficient for: 200 to 400 derivatizations• Component A: ATTO-TAG FQ, 5 mg• Component B: Mandelonitrile, 20 mM in 4 mL DMSO• Component C: β-cyclodextrin, 250 mgStore in freezer (-5°C to -30°C) and protect from light.
Form: __
GC-Rich PCR Performance: __
Polymerase: __
Technique: __
Kit Type: __
Quantity: 1 Kit
Target Organism Class: __
Purification Target: __
Sample Type: __
Detection Method: __
Format: Kit
PCR Method: __
Reaction Speed: __
For Use With (Equipment): __
Label or Dye: ATTO-TAG FQ
Species: __
For Use With (Application): __
Reaction Format: __
Starting Material: __
Content And Storage:
Sufficient for: 200 to 400 derivatizations• Component A: ATTO-TAG FQ, 5 mg• Component B: Mandelonitrile, 20 mM in 4 mL DMSO• Component C: β-cyclodextrin, 250 mgStore in freezer (-5°C to -30°C) and protect from light.
Form:
__
GC-Rich PCR Performance:
__
Polymerase:
__
Technique:
__
Kit Type:
__
Quantity:
1 Kit
Target Organism Class:
__
Purification Target:
__
Sample Type:
__
Detection Method:
__
Format:
Kit
PCR Method:
__
Reaction Speed:
__
For Use With (Equipment):
__
Label or Dye:
ATTO-TAG FQ
Species:
__
For Use With (Application):
__
Reaction Format:
__
Starting Material:
__
Content And Storage: Sufficient for: 300 to 800 assays• Component A: ATTO-TAG CBQCA derivatization reagent, 5 mg• Component B: Dimethylsulfoxide (DMSO), 1 mL• Component C: Mandelonitrile, 20 mM in 4 mL DMSO• Component D: Bovine serum albumin (BSA) standard, 4 mg• BupH Borate Buffer PackStore in freezer (-5°C to -30°C) and protect from light.
Form: __
GC-Rich PCR Performance: __
Polymerase: __
Technique: __
Kit Type: __
Quantity: 1 Kit
Target Organism Class: __
Purification Target: __
Sample Type: __
Detection Method: Fluorescence
Format: Kit
PCR Method: __
Reaction Speed: __
For Use With (Equipment): Microplate Reader, Fluorometer
Label or Dye: __
Species: __
For Use With (Application): Quantitating proteins in solution
Reaction Format: __
Starting Material: __
Content And Storage:
Sufficient for: 300 to 800 assays• Component A: ATTO-TAG CBQCA derivatization reagent, 5 mg• Component B: Dimethylsulfoxide (DMSO), 1 mL• Component C: Mandelonitrile, 20 mM in 4 mL DMSO• Component D: Bovine serum albumin (BSA) standard, 4 mg• BupH Borate Buffer PackStore in freezer (-5°C to -30°C) and protect from light.
Form:
__
GC-Rich PCR Performance:
__
Polymerase:
__
Technique:
__
Kit Type:
__
Quantity:
1 Kit
Target Organism Class:
__
Purification Target:
__
Sample Type:
__
Detection Method:
Fluorescence
Format:
Kit
PCR Method:
__
Reaction Speed:
__
For Use With (Equipment):
Microplate Reader, Fluorometer
Label or Dye:
__
Species:
__
For Use With (Application):
Quantitating proteins in solution
Reaction Format:
__
Starting Material:
__