Thermo Scientific™ ABsolute™ qPCR Mixes

Description

• ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes
• The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility
• ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye
• There is also a master mix version optimized for capillary instruments
• Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes
• The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility
• ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye
• There is also a master mix version optimized for capillary instruments
• Highlights • High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase • Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency • Optimised buffer - For maximal performance in multiplex reactions Applications • Gene expression analysis • siRNA validation • Genotyping Includes • Absolute qPCR Mix (no ROX) contains 2X Master Mix and no passive reference dye • Absolute qPCR ROX (500 nm) Mix contains 2X Master Mix supplemented with high ROX concentration • Absolute qPCR Low ROX Mix contains 2X Master Mix supplemented with low ROX concentration • Absolute qPCR Capillary Mix contains 2X Master Mix optimized for capillary instruments Hazardous:No > Storage Condition:-20°C > Storage notes:Note that ROX dye is light sensitive; exposure should be minimized
• Gene expression analysis, siRNA validation, and genotyping.