F160L
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F160L-Each-of-1 | In Stock | ₹ 84,832.74 |
F160L - Each of 1
In Stock
Quantity
1
Base Price: ₹ 84,832.74
GST (18%): ₹ 15,269.893
Total Price: ₹ 1,00,102.633
Content And Storage
• 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL• Dilution Buffer, 2 x 5 mL• 25 μM Control Primer Mix, 40 μL• Nuclease-free water, 10 x 1.25 mL• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)Store at -20°C.
PCR Method
Direct PCR
Reaction Speed
Fast
Shipping Condition
Wet or Dry Ice
Concentration
2X
No. of Reactions
1250 Reactions
Reaction Format
SuperMix or Master Mix
Starting Material
DNA
GC-Rich PCR Performance
Medium
Polymerase
Phire Hot Start II DNA Polymerase
Product Type
Direct PCR Master Mix
For Use With (Application)
Genotyping, Hot-start PCR
Hot Start
Built-In Hot Start
Overhang
Blunt
Sample Type
Plant
Related Products
Description
- Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples
- No DNA purification is required prior to PCR
- The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain
- The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others
- Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast
- The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA
- Features Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps Available Dilution & Storage protocol allows several PCR reactions from one sample Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel Applications Genotyping Transgene detection Knockout analysis Sequencing Notes Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq -based polymerases.
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Content And Storage: • 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL• Dilution Buffer, 2 x 5 mL• 25 μM Control Primer Mix, 40 μL• Nuclease-free water, 10 x 1.25 mL• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)Store at -20°C.
PCR Method: Direct PCR
Reaction Speed: Fast
Shipping Condition: Wet or Dry Ice
Concentration: 2X
No. of Reactions: 1250 Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: DNA
GC-Rich PCR Performance: Medium
Polymerase: Phire Hot Start II DNA Polymerase
Product Type: Direct PCR Master Mix
For Use With (Application): Genotyping, Hot-start PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: Plant
Content And Storage:
• 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL• Dilution Buffer, 2 x 5 mL• 25 μM Control Primer Mix, 40 μL• Nuclease-free water, 10 x 1.25 mL• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)Store at -20°C.
PCR Method:
Direct PCR
Reaction Speed:
Fast
Shipping Condition:
Wet or Dry Ice
Concentration:
2X
No. of Reactions:
1250 Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
DNA
GC-Rich PCR Performance:
Medium
Polymerase:
Phire Hot Start II DNA Polymerase
Product Type:
Direct PCR Master Mix
For Use With (Application):
Genotyping, Hot-start PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
Plant
Content And Storage: Contains: 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X), 2 x 5 mL Dilution Buffer, 40 μL Control Primer Mix (25 μM each), 10 x 1.25 mL Water, nuclease- free, 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications), Store at -20°C.
PCR Method: Direct PCR
Reaction Speed: Fast
Shipping Condition: __
Concentration: __
No. of Reactions: 1250 x 50 L Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: DNA
GC-Rich PCR Performance: Medium
Polymerase: Phire Hot Start II DNA polymerase
Product Type: Direct PCR Master Mix
For Use With (Application): Genotyping
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: Plant
Content And Storage:
Contains: 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X), 2 x 5 mL Dilution Buffer, 40 μL Control Primer Mix (25 μM each), 10 x 1.25 mL Water, nuclease- free, 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications), Store at -20°C.
PCR Method:
Direct PCR
Reaction Speed:
Fast
Shipping Condition:
__
Concentration:
__
No. of Reactions:
1250 x 50 L Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
DNA
GC-Rich PCR Performance:
Medium
Polymerase:
Phire Hot Start II DNA polymerase
Product Type:
Direct PCR Master Mix
For Use With (Application):
Genotyping
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
Plant
Content And Storage: • 2X Phire Plant Direct PCR Master Mix, 2 x 1.25 mL• Dilution Buffer, 1 x 5 mL• 25 μM Control Primer Mix, 40 μL• Nuclease-free water, 2 x 1.25 mL• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)Store at -20°C.
PCR Method: Direct PCR
Reaction Speed: Fast
Shipping Condition: Wet or Dry Ice
Concentration: 2X
No. of Reactions: 250 Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: DNA
GC-Rich PCR Performance: Medium
Polymerase: Phire Hot Start II DNA Polymerase
Product Type: Direct PCR Master Mix
For Use With (Application): Genotyping, Hot-start PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: Plant
Content And Storage:
• 2X Phire Plant Direct PCR Master Mix, 2 x 1.25 mL• Dilution Buffer, 1 x 5 mL• 25 μM Control Primer Mix, 40 μL• Nuclease-free water, 2 x 1.25 mL• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)Store at -20°C.
PCR Method:
Direct PCR
Reaction Speed:
Fast
Shipping Condition:
Wet or Dry Ice
Concentration:
2X
No. of Reactions:
250 Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
DNA
GC-Rich PCR Performance:
Medium
Polymerase:
Phire Hot Start II DNA Polymerase
Product Type:
Direct PCR Master Mix
For Use With (Application):
Genotyping, Hot-start PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
Plant
Content And Storage: Contains: 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X), 5 mL Dilution Buffer, 40 μL Control Primer Mix (25 μM each), 2 x 1.25 mL Water, nuclease- free, 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications), Store at -20°C.
PCR Method: Direct PCR
Reaction Speed: Fast
Shipping Condition: __
Concentration: __
No. of Reactions: 250 x 50 L Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: DNA
GC-Rich PCR Performance: Medium
Polymerase: Phire Hot Start II DNA polymerase
Product Type: Direct PCR Master Mix
For Use With (Application): Genotyping
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: Plant
Content And Storage:
Contains: 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X), 5 mL Dilution Buffer, 40 μL Control Primer Mix (25 μM each), 2 x 1.25 mL Water, nuclease- free, 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications), Store at -20°C.
PCR Method:
Direct PCR
Reaction Speed:
Fast
Shipping Condition:
__
Concentration:
__
No. of Reactions:
250 x 50 L Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
DNA
GC-Rich PCR Performance:
Medium
Polymerase:
Phire Hot Start II DNA polymerase
Product Type:
Direct PCR Master Mix
For Use With (Application):
Genotyping
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
Plant