Thermo Scientific™ DyNamo ColorFlash Probe qPCR Kit
Manufacturer: Thermo Scientific™
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Content And Storage
Contains:• 5 x 1 mL DyNAmo ColorFlash qPCR Mix (2X)• 1 x 250 μL 50X ROX dye, 25μM• 1 x 1000 μL 40X sample buffer solution with yellow dyeStore at -20°C
Format
Tube
PCR Method
qPCR
Reaction Speed
Fast
Kit Type
DyNAmo ColorFlash Probe qPCR Kit
Product Type
Real Time PCR SYBR Master Mix
For Use With (Application)
Gene Expression
No. of Reactions
500 Reactions
Volume
5 x 1 mL
Detection Method
Primer-probe
GC-Rich PCR Performance
High
Polymerase
Tbr DNA polymerase
For Use With (Equipment)
7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Stratagene Mx4000, StepOne™ System, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, BioRad MyiQ, BioRad iQ5, BioRad iCycler iQ, Cepheid SmartCycler, Stratagene Mx3005P
Product Line
DyNAmo Flash
Sufficient For
500 Reactions of 20 μL, 200 reactions of 50 μL
Concentration
2X (DyNAmo ColorFlash qPCR Mix), 50X (ROX Dye), 40X (Sample Buffer Solution with Yellow Dye)
Sample Type
DNA, dsDNA
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Description
- DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit
- In addition, it incorporates an innovative multicolor system that ensures correct pipetting
- The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye
- The qPCR reaction mix containing both components is green
- Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored
- This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels
- The dyes do not affect the specificity or sensitivity of qPCR assays
- Minimized risk of pipetting errors during reaction set up Especially beneficial when using white reaction vessels Extremely fast protocols due to combined annealing and extension step of only 15 sec
- Specific and sensitive detection of a wide range of template concentrations dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination
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Content And Storage: Contains:• 5 x 1 mL DyNAmo ColorFlash qPCR Mix (2X)• 1 x 250 μL 50X ROX dye, 25μM• 1 x 1000 μL 40X sample buffer solution with yellow dyeStore at -20°C
Format: Tube
PCR Method: qPCR
Reaction Speed: Fast
Kit Type: DyNAmo ColorFlash Probe qPCR Kit
Product Type: Real Time PCR SYBR Master Mix
For Use With (Application): Gene Expression
No. of Reactions: 500 Reactions
Volume: 5 x 1 mL
Detection Method: Primer-probe
GC-Rich PCR Performance: High
Polymerase: Tbr DNA polymerase
For Use With (Equipment): 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Stratagene Mx4000, StepOne™ System, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, BioRad MyiQ, BioRad iQ5, BioRad iCycler iQ, Cepheid SmartCycler, Stratagene Mx3005P
Product Line: DyNAmo Flash
Sufficient For: 500 Reactions of 20 μL, 200 reactions of 50 μL
Concentration: 2X (DyNAmo ColorFlash qPCR Mix), 50X (ROX Dye), 40X (Sample Buffer Solution with Yellow Dye)
Sample Type: DNA, dsDNA
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