A28007
Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit
Manufacturer: Applied Biosystems™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A28007-Each-of-1 | In Stock | ₹ 30,630.48 |
A28007 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 30,630.48
GST (18%): ₹ 5,513.486
Total Price: ₹ 36,143.966
Content And Storage
• 10X PolyA Buffer• ATP, 10mM• Poly A Enzyme, 5 U/μL• 5X Ligase Buffer• RNA Ligase, 10 U/μL• 50% PEG 8000• 25X Ligation Adaptor• 10X RT Enzyme Mix• 20X Universal RT Primer• dNTP Mix, 100mM• 20X miR-Amp Primer Mix• 5X RT Buffer• 2X miR-Amp Master MixStore all at -5 to -30°C, except 2X miR-Amp Master Mix which is stored at 2–8°C.
GC-Rich PCR Performance
High
Reaction Speed
Standard
Optimal Reaction Temperature
42°C
Product Type
cDNA Synthesis Kit
Reverse Transcriptase
SuperScript™ III
For Use With (Application)
miRNA analysis
Reaction Format
Separate Components
Volume
10 μL
Detection Method
Primer-probe
PCR Method
qPCR
For Use With (Equipment)
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line
TaqMan™
Quantity
50 cDNA synthesis reactions
Shipping Condition
Dry Ice
No. of Reactions
50 cDNA synthesis reactions / 600 qPCR reactions
Sample Type
RNA
Related Products
Description
- The Applied Biosystems TaqMan Advanced miRNA cDNA Synthesis Kit uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA Assays for detection and quantification of mature miRNAs in biological samples. The kit is designed to perform 50 cDNA synthesis reactions, with theresulting cDNA sufficient for up to 600 qPCR reactions, allowing extensive downstream analysis
- Features of the TaqMan Advanced miRNA workflow include: • Sensitivity: high sensitivity allows for quantification of low-expressing miRNA targets, especially in biological fluids such as plasma, serum, and tissue • Flexibility: the universal RT chemistry generates universal cDNA templates for all miRNAs present in the sample, allowing for flexibility in study design • Sample savings: requires only 2 μL of plasma/serum sample input and generates enough cDNA for 600 qPCR reactions (20 μL) or 1200 qPCR reactions (10 μL) • Specificity: 5’ ligation step allows for single base pair discrimination along entire miRNA sequence, including difficult-to-detect mismatches on 5’ end • Gold-standard TaqMan chemistry: This kit is designed for use with TaqMan Advanced miRNA Assays for qPCR, which draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and validation system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families
- The TaqMan Advanced miRNA cDNA Synthesis Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription
- Universal RT primers recognize the universal sequences present on both the 5' and 3' extended ends of the mature miRNAs
- All mature miRNAs in the sample are reverse transcribed to cDNA
- In order to improve detection of low-expressing miRNA targets, the cDNA is then amplified using the Universal miR-Amp Primers and miR-Amp Master Mix to uniformly increase the amount of cDNA for each target, maintaining the relative differential expression levels
- Unlike traditional pre-amplification, the Universal miR-Amp Primers recognize the universal sequences added to all mature miRNAs on the 5' and 3' ends, ensuring that there is no amplification bias
- Note: the TaqMan Advanced miRNA cDNA Synthesis Kit is not compatible with first generation TaqMan MicroRNA Assays for qPCR
- For reverse transcription of miRNA targets using miRNA-specific RT primers, please see the TaqMan MicroRNA Reverse Transcription Kit
- Order Info Note: the TaqMan Advanced miRNA cDNA Synthesis Kit is not compatible with first generation TaqMan MicroRNA Assays for qPCR
- For reverse transcription of miRNA targets using miRNA-specific RT primers
- Shipping Conditions: Dry Ice
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Content And Storage: • 10X PolyA Buffer• ATP, 10mM• Poly A Enzyme, 5 U/μL• 5X Ligase Buffer• RNA Ligase, 10 U/μL• 50% PEG 8000• 25X Ligation Adaptor• 10X RT Enzyme Mix• 20X Universal RT Primer• dNTP Mix, 100mM• 20X miR-Amp Primer Mix• 5X RT Buffer• 2X miR-Amp Master MixStore all at -5 to -30°C, except 2X miR-Amp Master Mix which is stored at 2–8°C.
GC-Rich PCR Performance: High
Reaction Speed: Standard
Optimal Reaction Temperature: 42°C
Product Type: cDNA Synthesis Kit
Reverse Transcriptase: SuperScript™ III
For Use With (Application): miRNA analysis
Reaction Format: Separate Components
Volume: 10 μL
Detection Method: Primer-probe
PCR Method: qPCR
For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line: TaqMan™
Quantity: 50 cDNA synthesis reactions
Shipping Condition: Dry Ice
No. of Reactions: 50 cDNA synthesis reactions / 600 qPCR reactions
Sample Type: RNA
Content And Storage:
• 10X PolyA Buffer• ATP, 10mM• Poly A Enzyme, 5 U/μL• 5X Ligase Buffer• RNA Ligase, 10 U/μL• 50% PEG 8000• 25X Ligation Adaptor• 10X RT Enzyme Mix• 20X Universal RT Primer• dNTP Mix, 100mM• 20X miR-Amp Primer Mix• 5X RT Buffer• 2X miR-Amp Master MixStore all at -5 to -30°C, except 2X miR-Amp Master Mix which is stored at 2–8°C.
GC-Rich PCR Performance:
High
Reaction Speed:
Standard
Optimal Reaction Temperature:
42°C
Product Type:
cDNA Synthesis Kit
Reverse Transcriptase:
SuperScript™ III
For Use With (Application):
miRNA analysis
Reaction Format:
Separate Components
Volume:
10 μL
Detection Method:
Primer-probe
PCR Method:
qPCR
For Use With (Equipment):
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line:
TaqMan™
Quantity:
50 cDNA synthesis reactions
Shipping Condition:
Dry Ice
No. of Reactions:
50 cDNA synthesis reactions / 600 qPCR reactions
Sample Type:
RNA
Content And Storage: Computer not Included
GC-Rich PCR Performance: __
Reaction Speed: __
Optimal Reaction Temperature: __
Product Type: __
Reverse Transcriptase: __
For Use With (Application): __
Reaction Format: __
Volume: __
Detection Method: __
PCR Method: __
For Use With (Equipment): QuantStudio™
Product Line: QuantStudio™
Quantity: 1 System
Shipping Condition: Room Temperature
No. of Reactions: __
Sample Type: __
Content And Storage:
Computer not Included
GC-Rich PCR Performance:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Product Type:
__
Reverse Transcriptase:
__
For Use With (Application):
__
Reaction Format:
__
Volume:
__
Detection Method:
__
PCR Method:
__
For Use With (Equipment):
QuantStudio™
Product Line:
QuantStudio™
Quantity:
1 System
Shipping Condition:
Room Temperature
No. of Reactions:
__
Sample Type:
__
Content And Storage: Computer not Included
GC-Rich PCR Performance: __
Reaction Speed: __
Optimal Reaction Temperature: __
Product Type: __
Reverse Transcriptase: __
For Use With (Application): __
Reaction Format: __
Volume: __
Detection Method: __
PCR Method: __
For Use With (Equipment): QuantStudio™
Product Line: QuantStudio™
Quantity: 1 system
Shipping Condition: Room Temperature
No. of Reactions: __
Sample Type: __
Content And Storage:
Computer not Included
GC-Rich PCR Performance:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Product Type:
__
Reverse Transcriptase:
__
For Use With (Application):
__
Reaction Format:
__
Volume:
__
Detection Method:
__
PCR Method:
__
For Use With (Equipment):
QuantStudio™
Product Line:
QuantStudio™
Quantity:
1 system
Shipping Condition:
Room Temperature
No. of Reactions:
__
Sample Type:
__
Content And Storage: __
GC-Rich PCR Performance: __
Reaction Speed: __
Optimal Reaction Temperature: __
Product Type: __
Reverse Transcriptase: __
For Use With (Application): __
Reaction Format: __
Volume: __
Detection Method: __
PCR Method: __
For Use With (Equipment): __
Product Line: __
Quantity: __
Shipping Condition: __
No. of Reactions: __
Sample Type: __
Content And Storage:
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Product Type:
__
Reverse Transcriptase:
__
For Use With (Application):
__
Reaction Format:
__
Volume:
__
Detection Method:
__
PCR Method:
__
For Use With (Equipment):
__
Product Line:
__
Quantity:
__
Shipping Condition:
__
No. of Reactions:
__
Sample Type:
__