Applied Biosystems™ TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, standard

Description

• This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples
• Two exogenous (non-human) controls are included for normalization of sample input
• Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include: • Reproducibility —pre-spotted assays are designed to reduce pipetting error and minimize experimental variability • Sample savings —requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample • Updated content —assay content updated for mirBase v.21 • Easy to use —just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required • Gold-standard TaqMan qPCR chemistry —TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families Product details: Number of plates: 1 Number of unique miRNAs (not including exogenous controls): 30 Exogenous controls: cel-miR-39-3p, ath-miR159a Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex Recommended master mix: TaqMan Fast Advanced Master Mix Notes • TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples
• • These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.