Thermo Scientific™ Phusion Site-Directed Mutagenesis Kit PROMO

Description

• Robust and reliable exponential amplification method No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid No need to destroy the starting template in a separate step Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations Amplification of large plasmids up to 10 kb Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR T4 DNA Ligase included in the kit; no purification steps before or after ligation Compatible with all strains of competent E
• coli cells