Thermo Scientific™ Mutation Generation System Kit

Description

• Description transposon products are based on the transposition machinery of the bacteriophage Mu
• During the lytic phase of the phage's life cycle the machinery replicates its genome by transposing repeatedly inside the host genome
• The Mu transposition reaction has been modified into an in vitro reaction catalyzed by a single enzyme - MuA Transposase
• In this system, one in vitro reaction is capable of generating more than a million transposon insertion clones
• Highlights The Mutation Generation System (MGS Kit) and Stop Generation System (STOP Kit) were developed for functional analysis of proteins
• These new transposon tools enable the creation of saturated libraries of mutated proteins in a single reaction with less hands-on time than any other method
• The location of the transposon insertion in each mutated clone can be mapped by either PCR or sequencing
• With MGS and STOP kits, thousands of mutated clones are ready for expression studies in just 2 to 3 days
• The MGS Kit contains the complete set of reagents for transposon-based linker scanning mutagenesis of any target protein
• The MGS Entranceposons are designed for making subtle changes in the structure of a target protein by inserting 15 bp in-frame linkers throughout the corresponding target gene
• This in-frame insertion allows for conservation of downstream sequences
• The STOP Kit Entranceposons contain translational stop codons in all three reading frames within the terminal portion of the transposon sequence
• The proprietary modification of the Stop Generation System makes it possible to generate a saturated C-terminal deletion library from virtually any target protein with a maximum addition of three amino acids
• Highlights Efficient - Create saturated insertion libraries for sequencing and protein analysis in a single reaction Fast - Decrease hands-on time compared to conventional methods Random - Eliminate target site preference or insertion hot-spot